Structure of two human alpha-tubulin genes.
Structure of two human alpha-tubulin genes.
复制标题
两个人类α-微管蛋白基因的结构。
DOI：
10.1073/pnas.79.1.96
复制
发表时间：
1982
影响因子：
11.1
通讯作者：
Cowan,NJ
中科院分区：
文献类型：
--
作者：
Wilde,CD;Chow,LT;Wefald,FC;Cowan,NJ
鸡α-微管蛋白cDNA探针在严格条件下与人DNA交叉杂交的能力已被用于筛选两个独立构建的人基因组文库。分离出9个克隆，占在人DNA的全基因组Southern印迹中观察到的条带的60%。选择两个克隆用于通过限制性作图、使用3 '-或5'-特异性探针的定向实验和异源双链体的电子显微镜进一步分析。一个克隆，2 α，含有α-微管蛋白特异性区域的5.0激酶，包括三个插入序列。第二个克隆，19 α，含有5.4个微管蛋白酶的α-微管蛋白特异性区域，并具有稍微分叉的5'和3'末端。克隆19 α仅具有两个间插序列，其对应于克隆2 α中的前两个。然而，这些插入序列在克隆2 α和19 α之间大小不同，并且没有显示出可检测的序列同源性。与cDNA探针杂交的任一克隆中的序列长度之和基本上占cDNA分子的整个长度。
The ability of a chicken alpha-tubulin cDNA probe to cross-hybridize with human DNA under stringent conditions has been exploited to screen two independently constructed human genomic libraries. Nine clones were isolated, accounting for 60% of the bands observed in a whole genomic Southern blot of human DNA. Two clones were selected for further analysis by restriction mapping, orientation experiments using 3'- or 5'-specific probes, and electron microscopy of heteroduplexes. One clone, 2 alpha, contains an alpha-tubulin-specific region of 5.0 kilobases that includes three intervening sequences. The second clone, 19 alpha, contains an alpha-tubulin-specific region of 5.4 kilobases and has somewhat diverged 5' and 3' ends. Clone 19 alpha has only two intervening sequences that correspond to the first two in clone 2 alpha. However, these intervening sequences differ in size between clones 2 alpha and 19 alpha and show no detectable sequence homology. The sum of the lengths of sequences in either clone that hybridize to the cDNA probe accounts for essentially the entire length of the cDNA molecule.