Evaluation of laser diode thermal desorption-tandem mass spectrometry (LDTD-MS-MS) in forensic toxicology.
Evaluation of laser diode thermal desorption-tandem mass spectrometry (LDTD-MS-MS) in forensic toxicology.
复制标题
激光二极管热解吸串联质谱 (LDTD-MS-MS) 在法医毒理学中的评估。
DOI：
10.1093/jat/bku084
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发表时间：
2014
影响因子：
2.5
通讯作者：
Megan Grabenauer
中科院分区：
文献类型：
--
作者：
Nichole Bynum;K. Moore;Megan Grabenauer
由于与毒品有关的案件增加，许多法医实验室的工作积压。激光二极管热解吸（LDTD）已证明其在其他科学领域的适用性，提供的数据与仪器，如液相色谱串联质谱，在更短的时间。LDTD-MS-MS用于验证无药物的人尿液和血液中的48种化合物，用于筛选或定量分析。评价了残留、干扰、检测限、定量限、基质效应、线性、精密度、准确度和稳定性。定量分析表明，LDTD-MS-MS产生了精确和准确的结果，尿液和血液中的平均总体运行内精密度分别表示为%CV <14.0和<7.0。尿液中所有药物的准确度范围为88.9 - 104.5%，血液中为91.9 - 107.1%。总体而言，LDTD具有用于法医毒理学的潜力，但在成功实施之前，必须解决一些挑战。虽然LDTD系统的优点包括最低限度的维护和快速分析（每个样品10秒），这使其成为高通量法医实验室的理想选择，但其主要缺点是由于缺乏色谱而不使用高分辨率MS，因此无法或难以分析异构体和同量异位素;因此，它最好作为筛选技术实施。
Many forensic laboratories experience backlogs due to increased drug-related cases. Laser diode thermal desorption (LDTD) has demonstrated its applicability in other scientific areas by providing data comparable with instrumentation, such as liquid chromatography-tandem mass spectrometry, in less time. LDTD-MS-MS was used to validate 48 compounds in drug-free human urine and blood for screening or quantitative analysis. Carryover, interference, limit of detection, limit of quantitation, matrix effect, linearity, precision and accuracy and stability were evaluated. Quantitative analysis indicated that LDTD-MS-MS produced precise and accurate results with the average overall within-run precision in urine and blood represented by a %CV <14.0 and <7.0, respectively. The accuracy for all drugs in urine ranged from 88.9 to 104.5% and 91.9 to 107.1% in blood. Overall, LDTD has the potential for use in forensic toxicology but before it can be successfully implemented that there are some challenges that must be addressed. Although the advantages of the LDTD system include minimal maintenance and rapid analysis (∼10 s per sample) which makes it ideal for high-throughput forensic laboratories, a major disadvantage is its inability or difficulty analyzing isomers and isobars due to the lack of chromatography without the use of high-resolution MS; therefore, it would be best implemented as a screening technique.
影响因子：
7.4
作者：
Matuszewski, BK;Constanzer, ML;Chavez-Eng, CM
通讯作者：
Chavez-Eng, CM