Recent advances in integrated solid-state nanopore sensors.
Recent advances in integrated solid-state nanopore sensors.
复制标题
集成固态纳米孔传感器的最新进展。
DOI：
10.1039/d1lc00294e
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发表时间：
2021-08-21
期刊：
影响因子：
6.1
通讯作者：
Schmidt H
中科院分区：
文献类型：
--
作者：
Rahman M;Sampad MJN;Hawkins A;Schmidt H
单分子探测技术的出现彻底改变了生物医学和生命科学领域，并刺激了基于强大生物传感器的新型芯片实验室的发展。纳米孔代表了最新和最有前途的单分子传感范例之一，其正在增加芯片级集成以提高便利性和性能。由于它们的物理结构，纳米孔是高度敏感的，需要低样品体积，并提供无标记，无扩增，高通量实时检测和鉴定生物分子。在过去的25年中，纳米孔已被广泛用于检测各种生物分子，其应用范围从核酸测序到超灵敏诊断再到单分子生物物理学。纳米孔，特别是固态膜中的纳米孔，也有可能与其他技术集成，如光学、等离子体、微流体和光流体，以执行更复杂的任务，满足不断扩大的需求。已经报道了一些使用集成纳米孔平台的突破性结果，并且可以预期更多的纳米孔仍然是创新研究的焦点，并且正在进入商业仪器。这篇综述概述了纳米孔技术的不同方面和挑战，重点是固态纳米孔在生物传感和生物分析应用中的芯片级集成。纳米孔是功能强大的单分子传感器，具有从单分子生物物理学到医学诊断的广泛应用。这篇评论涵盖了纳米孔传感器集成到日益复杂的芯片实验室系统的各个方面。
The advent of single-molecule probing techniques has revolutionized the biomedical and life science fields and has spurred the development of a new class of labs-on-chip based on powerful biosensors. Nanopores represent one of the most recent and most promising single molecule sensing paradigms that is seeing increased chip-scale integration for improved convenience and performance. Due to their physical structure, nanopores are highly sensitive, require low sample volume, and offer label-free, amplification-free, high-throughput real-time detection and identification of biomolecules. Over the last 25 years, nanopores have been extensively employed to detect a variety of biomolecules with a growing range of applicatons ranging from nucleic acid sequencing to ultrasensitive diagnostics to single-molecule biophysics. Nanopores, in particular those in solid-state membranes, also have the potential for integration with other technologies such as optics, plasmonics, microfluidics, and optofluidics to perform more complex tasks for an ever-expanding demand. A number of breakthrough results using integrated nanopore platforms have already been reported, and more can be expected as nanopores remain the focus of innovative research and are finding their way into commercial instruments. This review provides an overview of different aspects and challenges of nanopore technology with a focus on chip-scale integration of solid-state nanopores for biosensing and bioanalytical applications. Nanopores are powerful single molecule sensors that have a wide range of applications from single molecule biophysics to medical diagnostics. This review covers all aspects of nanopore sensor integration into increasingly complex lab-on-chip systems.
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影响因子：
8.6
作者：
ASHKIN, A
通讯作者：
ASHKIN, A
影响因子：
2.8
作者：
Bello, Julian;Shim, Jiwook
通讯作者：
Shim, Jiwook
影响因子：
10.8
作者：
Angeli, Elena;Volpe, Andrea;Fanzio, Paola;Repetto, Luca;Firpo, Giuseppe;Guida, Patrizia;Lo Savio, Roberto;Wanunu, Meni;Valbusa, Ugo
通讯作者：
Valbusa, Ugo
影响因子：
13.3
作者：
Astier, Yann;Uzun, Oktay;Stellacci, Francesco
通讯作者：
Stellacci, Francesco
影响因子：
3.4
作者：
Bates, M;Burns, M;Meller, A
通讯作者：
Meller, A