Segregation Versus Colocalization: Orthogonally Functionalized Binary Micropatterned Substrates Regulate the Molecular Distribution in Focal Adhesions
Segregation Versus Colocalization: Orthogonally Functionalized Binary Micropatterned Substrates Regulate the Molecular Distribution in Focal Adhesions
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DOI：
10.1002/adma.201500900
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发表时间：
2015-07-01
影响因子：
29.4
通讯作者：
Spatz, Joachim P.
中科院分区：
文献类型：
--
作者：
Guasch, Judith;Conings, Bert;Spatz, Joachim P.
正交功能化二元微图案化衬底采用一种新的方案。使用足够的肽模拟物可以使纯化的α v β 3和α 5 β 1整合素在邻近的微岛上空前分离，并证明U2OS细胞倾向于将这些受体共定位。此外，这种趋势可以通过改变微图案的几何形状和组成来改变。
Orthogonally functionalized binary micropatterned substrates are produced using a novel protocol. The use of adequate peptido-mimetics enables an unprecedented segregation of purified alpha v beta 3 and alpha 5 beta 1 integrins in adjacent microislands and evidences the preference of U2OS cells to colocalize such receptors. Moreover, this tendency can be altered by varying the geometry and composition of the micropatterns.