Ultraviolet-ozone treatment reduces levels of disease-associated prion protein and prion infectivity.
Ultraviolet-ozone treatment reduces levels of disease-associated prion protein and prion infectivity.
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DOI：
10.1186/1756-0500-2-121
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发表时间：
2009-07-06
影响因子：
1.8
通讯作者：
Aiken, Judd M
中科院分区：
文献类型：
--
作者：
Johnson, Christopher J;Gilbert, P U P A;Aiken, Judd M
背景技术背景：传染性海绵状脑病（Transmissible spongiform encephalopathies，TSE）是一组由朊病毒引起的致命性神经退行性疾病。朊病毒似乎主要（如果不是唯一的话）由细胞朊病毒蛋白的错误折叠同种型组成。TSE传染性是非常稳定的，可以抵抗许多侵略性的去污程序，增加人类，牲畜和野生动物暴露于TSEs.FINDINGS：我们测试的假设，紫外线臭氧治疗降低致病性朊病毒蛋白的水平和灭活的传染性病原体。我们发现，紫外线-臭氧处理降低了感染的脑匀浆中的碳和朊病毒蛋白质含量，分别通过干灰化碳分析或免疫印迹检测不到的水平。在灰化8周后，UV-臭氧处理将经处理的材料的感染性滴度降低至少105倍。少量的传染性，然而，尽管紫外线臭氧治疗坚持。当绑定到蒙脱石粘土或石英表面，PrPTSE仍然容易降解UV-ozone.CONCLUSION：我们的研究结果强烈表明，UV-臭氧处理可以降解致病性朊病毒蛋白和朊病毒，即使当该试剂与表面。使用更大的紫外线-臭氧剂量或将紫外线-臭氧处理与其他去污方法相结合，可以对TSE污染的材料进行灭菌。
BACKGROUND: Transmissible spongiform encephalopathies (TSEs) are a group of fatal neurodegenerative diseases caused by novel infectious agents referred to as prions. Prions appear to be composed primarily, if not exclusively, of a misfolded isoform of the cellular prion protein. TSE infectivity is remarkably stable and can resist many aggressive decontamination procedures, increasing human, livestock and wildlife exposure to TSEs.FINDINGS: We tested the hypothesis that UV-ozone treatment reduces levels of the pathogenic prion protein and inactivates the infectious agent. We found that UV-ozone treatment decreased the carbon and prion protein content in infected brain homogenate to levels undetectable by dry-ashing carbon analysis or immunoblotting, respectively. After 8 weeks of ashing, UV-ozone treatment reduced the infectious titer of treated material by a factor of at least 105. A small amount of infectivity, however, persisted despite UV-ozone treatment. When bound to either montmorillonite clay or quartz surfaces, PrPTSE was still susceptible to degradation by UV-ozone.CONCLUSION: Our findings strongly suggest that UV-ozone treatment can degrade pathogenic prion protein and inactivate prions, even when the agent is associated with surfaces. Using larger UV-ozone doses or combining UV-ozone treatment with other decontaminant methods may allow the sterilization of TSE-contaminated materials.