Prevalence of autoantibodies to thyroglobulin in dogs with nonthyroidal illness.
Prevalence of autoantibodies to thyroglobulin in dogs with nonthyroidal illness.
复制标题
患有非甲状腺疾病的狗体内甲状腺球蛋白自身抗体的患病率。
DOI：
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发表时间：
1998
影响因子：
1
通讯作者：
Watson Gl
中科院分区：
文献类型：
--
作者：
Raymond J. Nachreiner;K. Refsal;P. Graham;Hauptman Jg;Watson Gl
目的
评估甲状腺球蛋白自身抗体（TgAA）测定并确定诊断阈值。
样本群体
血清样本来自各种内分泌异常犬和30只肥胖成年雌性比格犬。
程序
用ELISA法测定血清TgAA。进行了六个实验：1，使用正常和T3自身抗体（T3 AA）阳性犬的样本定义TgAA阳性结果; 2，在91只临床正常犬中确定阳性结果的发生率; 3，评价患有非甲状腺疾病的犬血清的阳性结果; 4，检测患有原发性甲状腺功能减退但不存在T4 AA或T3 AA或两者的犬的样本; 5，确定T4 AA和/或T3 AA阳性的狗中假阴性结果的发生率，所述狗（18只狗）或未（22只狗）接受L-甲状腺素替代疗法;和6，检查来自18只狗的甲状腺活检标本（8只TgAA阳性和10只TgAA阴性）。
结果
阳性结果定义为阴性对照样品光密度的至少两倍（200%）。146只患有非甲状腺疾病的犬中，假阳性结果仅为3.4%。37%患有原发性甲状腺功能减退症但没有T4 AA或T3 AA或两者的证据的狗是TgAA阳性。假阴性结果分别在1/22和2/18 T3 AA阳性犬与甲状腺替代治疗，没有。甲状腺活检标本从8 TgAA阳性犬淋巴细胞性甲状腺炎的证据，而从10 TgAA阴性犬没有。
结论和临床意义
该检测方法对犬淋巴细胞性自身免疫性甲状腺炎的鉴别具有灵敏度和特异性，并有可能帮助犬甲状腺炎的早期诊断，并在育种计划中鉴别可能持续甲状腺功能减退的犬。
OBJECTIVE
To evaluate a thyroglobulin autoantibody (TgAA) assay and determine a diagnostic threshold.
SAMPLE POPULATION
Serum samples from dogs with various endocrine abnormalities and from 30 obese adult female Beagles.
PROCEDURE
TgAA were determined by use of the ELISA. Six experiments were done: 1, definition of positive results for TgAA using samples from normal and T3 autoantibody (T3AA) positive dogs; 2, establishment of prevalence of positive results in 91 clinically normal dogs; 3, evaluation of positive results for sera from dogs with nonthyroidal illnesses; 4, testing of samples from dogs with primary hypothyroidism but absence of T4AA or T3AA, or both; 5, determination of prevalence of false-negative results in dogs that are T4AA and/or T3AA positive, which were (18 dogs) or were not (22 dogs) receiving L-thyroxine replacement therapy; and 6, examination of thyroid biopsy specimens from 18 dogs (8 TgAA positive and 10 TgAA negative).
RESULTS
Positive results were defined as at least twice (200%) the optical density of the negative-control sample. False-positive results were obtained for only 3.4% of 146 dogs with nonthyroidal illness. Thirty-seven percent of dogs with primary hypothyroidism, but no evidence of T4AA or T3AA, or both, were TgAA positive. False-negative results were found in 1 of 22 and 2 of 18 T3AA-positive dogs with and without thyroid replacement therapy, respectively. Thyroid biopsy specimens from 8 TgAA-positive dogs had evidence of lymphocytic thyroiditis, whereas those from 10 TgAA-negative dogs did not.
CONCLUSION AND CLINICAL RELEVANCE
The assay is sensitive and specific for identification of lymphocytic autoimmune thyroiditis in dogs, and has potential for aiding early diagnosis of thyroiditis in dogs and identifying dogs likely to perpetuate hypothyroidism in breeding programs.