CORONAVIRUS IBV - REMOVAL OF SPIKE GLYCOPOLYPEPTIDE-S1 BY UREA ABOLISHES INFECTIVITY AND HEMAGGLUTINATION BUT NOT ATTACHMENT TO CELLS
CORONAVIRUS IBV - REMOVAL OF SPIKE GLYCOPOLYPEPTIDE-S1 BY UREA ABOLISHES INFECTIVITY AND HEMAGGLUTINATION BUT NOT ATTACHMENT TO CELLS
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DOI：
10.1099/0022-1317-67-7-1443
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发表时间：
1986-07-01
影响因子：
3.8
通讯作者：
DAVIS, PJ
中科院分区：
文献类型：
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作者：
CAVANAGH, D;DAVIS, PJ
尿素已被用于去除鸡传染性支气管炎病毒（IBV）株M41和Beaudette的S1刺突糖肽，而不去除S2刺突锚定糖肽。pH值降至2.9不会导致S1的释放，尽管在pH 7.4时IBV Beaudette自发释放了一些S1。缺乏S1的病毒不再具有感染性或能够引起血凝（HA）。然而，缺乏S1的放射性标记的IBV附着于红细胞和鸡肾细胞的程度与完整病毒相同或相似。用磷脂酶C制剂处理IBV（使IBV引起HA所需的）并不增加IBV与红细胞的结合。结果表明，虽然缺乏S1的病毒对细胞的附着与完整病毒的附着有质的不同，但感染性的下降是其他一些刺突功能丧失的结果。
Urea has been used to remove the S1 spike glycopolypeptide from avian infectious bronchitis virus (IBV) strains M41 and Beaudette, without removing the S2 spike-anchoring glycopolypeptide. Reduction of the pH to 2.9 did not cause release of S1 although some S1 was released spontaneously from IBV Beaudette at pH 7.4. Virus that lacked S1 was no longer infectious or able to cause haemagglutnination (HA). However, radiolabelled IBV that lacked S1 attached to erythrocytes and chick kidney cells to the same or similar extent as did intact virus. Treatment of IBV with a phospholipase C preparation, required to make IBV cause HA, did not increase binding of IBV to erythrocytes. The results indicate that while the attachment to cells of virus that lacks S1 is qualitatively different from that of intact virus, the decline in infectivity is the consequence of the loss of some other spike function.