Effect of ribosome shielding on mRNA stability
Effect of ribosome shielding on mRNA stability
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DOI：
10.1088/1478-3975/10/4/046008
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发表时间：
2013-08-01
期刊：
影响因子：
2
通讯作者：
Valleriani, Angelo
中科院分区：
文献类型：
--
作者：
Deneke, Carlus;Lipowsky, Reinhard;Valleriani, Angelo
基于翻译核糖体稳定mRNA的实验证据，我们引入并研究了核糖体对mRNA动态屏蔽的理论模型。我们提出了一个改进的拟合发表的衰变测定数据在E。大肠杆菌中，并显示只有三分之一的衰减模式是指数。我们对平均寿命和mRNA半衰期的新的全转录组估计表明，这些时间尺度比以前的估计要短得多。我们还解释了为什么有一个负相关的mRNA长度和平均寿命时，mRNA被细分在类共享相同的降解参数。作为一个副产品，我们的模型表明，在E。大肠杆菌可能比以前认为的要少。
Based on the experimental evidence that translating ribosomes stabilize the mRNAs, we introduce and study a theoretical model for the dynamic shielding of mRNA by ribosomes. We present an improved fitting of published decay assay data in E. coli and show that only one third of the decay patterns are exponential. Our new transcriptome-wide estimate of the average lifetimes and mRNA half-lives shows that these timescales are considerably shorter than previous estimates. We also explain why there is a negative correlation between mRNA length and average lifetime when the mRNAs are subdivided in classes sharing the same degradation parameters. As a by-product, our model indicates that co-transcriptional translation in E. coli may be less common than previously believed.