Effect of mechanical stress on differentiation of mouse mesenchymal stem cells seeded into an octacalcium phosphate-gelatin scaffold
Effect of mechanical stress on differentiation of mouse mesenchymal stem cells seeded into an octacalcium phosphate-gelatin scaffold
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机械应力对接种到磷酸八钙明胶支架中的小鼠间充质干细胞分化的影响
DOI：
10.1016/j.snb.2015.05.073
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
O. Suzuki
中科院分区：
文献类型：
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作者：
M. Yamada;T. Anada;T. Masuda;T. Takano-Yamamoto;O. Suzuki
我们报告了生物活性三维支架上的机械应力的程度，该支架可以在体外诱导接种的成骨细胞的分化。将小鼠间充质干 D1 细胞接种在由磷酸八钙 (OCP) 和明胶 (Gel) 复合材料组成的多孔海绵支架上，该支架此前已被证明是一种足够的体内骨替代材料。使用为本研究设计的循环机械加载装置，通过使 5 毫米环形、细胞接种的 OCP/凝胶复合材料在 1-3 毫米（相当于材料厚度的 20-60%）范围内变形，进行单轴压缩加载后确定细胞活性。变形的程度不影响增殖。然而，在复合材料仅变形 20% 后，分化标记基因（例如骨桥蛋白和骨粘素）就会增强。这些结果表明，如果提供合适的机械应力，D1细胞的成骨细胞分化就会得到促进，这表明该材料在机械应力下的骨再生特性的生物相关性，这在之前的体内研究中得到了证明。
We report the extent of mechanical stress on a bioactive three-dimensional scaffold that can induce differentiation of seeded osteoblastic cells in vitro. Mouse mesenchymal stem D1 cells were seeded on a porous spongy scaffold consists of octacalcium phosphate (OCP) and gelatin (Gel) composite, which was previously proven to be a sufficient bone-replaceable material in vivo. The cellular activity was determined after loading of uni-axial compression by deforming a 5 mm ring-shaped, cell-seeded OCP/Gel composite in the range of 1–3 mm (corresponding to 20–60% of the material thickness) using a cyclic mechanical loading device designed for this study. The extent of the deformation did not affect the proliferation. However, enhancement of differentiation marker genes, such as osteopontin and osteoclacin, occurred after the composite was deformed by just 20%. These results suggest that osteoblastic differentiation of D1 cells is promoted if suitable mechanical stress is provided, which indicates biological relevance of the bone regenerative properties of this material under mechanical stress that were shown in a previous in vivo study.