PCR detection of type I polyketide synthase genes in myxobacteria
PCR detection of type I polyketide synthase genes in myxobacteria
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DOI：
10.1128/aem.00224-08
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发表时间：
2008-09-01
影响因子：
4.4
通讯作者：
Harayama, Shigeaki
中科院分区：
文献类型：
--
作者：
Komaki, Hisayuki;Fudou, Ryosuke;Harayama, Shigeaki
通过PCR扩增粘细菌中编码酮合酶和酰基转移酶结构域的DNA，研究了粘细菌I型模块化聚酮合酶（PKS）的多样性。扩增子的测序显示，许多PKS基因与已发表的序列有较远的相关性。因此，粘细菌可能是新型和多样的聚酮化合物的优良资源。
The diversity of type I modular polyketide synthase (PKS) was explored by PCR amplification of DNA encoding ketosynthase and acyltransferase domains in myxobacteria. The sequencing of the amplicons revealed that many PKS genes were distantly related to the published sequences. Thus, myxobacteria may be excellent resources for novel and diverse polyketides.