Identification and characterization of an antimicrobial peptide, lysozyme, from Suncus murinus.
Identification and characterization of an antimicrobial peptide, lysozyme, from Suncus murinus.
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来自 Suncus murinus 的抗菌肽溶菌酶的鉴定和表征。
DOI：
10.1007/s00441-019-02991-2
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Sakata I.
中科院分区：
文献类型：
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作者：
Takemi S;Ojima S;Tanaka T;Sakai T;Sakata I.
溶菌酶是最重要的抗菌肽之一，已从许多哺乳动物中鉴定出来。然而，这种酶还没有在食虫目中进行过研究，其中包括最原始的胎盘哺乳动物。在这里，我们做了溶菌酶的cDNA从Suncus murinus（简称为suncus，它的实验室名称）和比较预测的氨基酸序列从其他哺乳动物物种。定量PCR分析表明，该基因在梭子鱼的脾脏和胃肠道中有较高的表达。溶菌酶免疫阳性细胞主要分布于脾脏红髓和整个小肠粘膜，包括淋巴集结的滤泡相关上皮和上皮下穹隆。小肠中的溶菌酶-ip细胞主要分布在肠腺中，但溶菌酶表达细胞不仅存在于肠腺中，也存在于小肠绒毛中。另一方面，只有少数溶菌酶-ip细胞被发现在绒毛和一些颗粒，显示强烈的荧光位于管腔。如对其他哺乳动物所报道的，Ki 67-ip细胞位于隐窝中，并且不与溶菌酶-ip细胞共定位。此外，禁食诱导的溶菌酶在小肠的mRNA水平的下降。综上所述，本研究首次鉴定了溶菌酶mRNA序列，阐明了溶菌酶转录本在梭子鱼肠道中的表达模式，发现了梭子鱼肠道中溶菌酶产生细胞的独特分布。
Lysozyme is one of the most prominent antimicrobial peptides and has been identified from many mammalian species. However, this enzyme has not been studied in the order Insectivora, which includes the most primitive placental mammals. Here, we done the lysozyme cDNA fromSuncus murinus(referred to as suncus, its laboratory name) and compare the predicted amino acid sequence to those from other mammalian species. Quantitative PCR analysis revealed a relatively higher expression of this gene in the spleen and gastrointestinal tract of suncus. The lysozyme-immunopositive (ip) cells were found mainly in the red pulp of the spleen and in the mucosa of the whole small intestine, including the follicle-associated epithelium and subepithelial dome of Peyer’s patches. The lysozyme-ip cells in the small intestine were mostly distributed in the intestinal crypt, although lysozyme-expressing cells were found not only in the crypt but also in the villi. On the other hand, only a few lysozyme-ip cells were found in the villi and some granules showing intense fluorescence were located toward the lumen. As reported for other mammals, Ki67-ip cells were localized in the crypt and did not co-localize with the lysozyme-ip cells. Moreover, fasting induced a decrease in the mRNA levels of lysozyme in the intestine of suncus. In conclusion, we firstly identified the lysozyme mRNA sequence, clarified expression profile of lysozyme transcripts in suncus and found a unique distribution of lysozyme-producing cells in the suncus intestine.