SUB-UNIT STRUCTURE OF SOLUBLE AND MITOCHONDRIAL MALIC ENZYME - DEMONSTRATION OF HUMAN MITOCHONDRIAL ENZYME IN HUMAN-MOUSE HYBRIDS
SUB-UNIT STRUCTURE OF SOLUBLE AND MITOCHONDRIAL MALIC ENZYME - DEMONSTRATION OF HUMAN MITOCHONDRIAL ENZYME IN HUMAN-MOUSE HYBRIDS
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DOI：
10.1111/j.1469-1809.1975.tb00123.x
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发表时间：
1975-01-01
影响因子：
1.9
通讯作者：
BUCKTON, KE
中科院分区：
文献类型：
--
作者：
POVEY, S;WILSON, DE;BUCKTON, KE
1.用淀粉凝胶电泳法检测了培养的人成纤维细胞和60例脑组织中的苹果酸酶。MEM的多态性已被证实，电泳图谱表明，这种酶，像MES，是一个四聚体。2.可溶性和线粒体苹果酸酶在小鼠和中国仓鼠的外观进行了描述，并在人，小鼠和仓鼠的酶的身份已建立从种间体细胞杂交的研究。在人类和仓鼠中，MES将阳极迁移到MEM，而在小鼠中，MES的阳极迁移相对少于MEM。人-小鼠和人-仓鼠体细胞杂交体中人酶的分离证实了MES和PGM 3的同线性。来自人-小鼠杂交的数据表明，MEM可能与MES不同线，可能与人的MES不同线，也与GPI不同线。
1. Malic enzyme has been examined by starch-gel electrophoresis in human cultured fibroblasts and in 60 brain samples. The polymorphism of MEM has been confirmed and the electrophoretic pattern suggests that this enzyme, like MES, is a tetramer. 2. The appearance of the soluble and mitochondrial malic enzymes in mouse and Chinese hamster is described, and the identity of the enzymes in man, mouse and hamster has been established from a study of interspecific somatic cell hybrids. In man and in hamster MES migrates anodal to MEM, whereas in the mouse MES is relatively less anodal than MEM. 3. Segregation of human enzymes in human--mouse and human--hamster somatic cell hybrids confirms the synteny of MES and PGM3. Data from human--mouse hybrids suggest that MEM is probably not syntenic with MES is probably not syntenic with MES in man, nor is it syntenic with GPI.