Chemical stress-responsive genes from the lignin-degrading fungus Phanerochaete chrysosporium exposed to dibenzo-p-dioxin.
Chemical stress-responsive genes from the lignin-degrading fungus Phanerochaete chrysosporium exposed to dibenzo-p-dioxin.
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来自暴露于二苯并-对-二恶英的木质素降解真菌黄孢原毛平革菌的化学胁迫响应基因。
DOI：
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发表时间：
2002
影响因子：
2.1
通讯作者：
Hiroo Tanaka
中科院分区：
文献类型：
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作者：
H. Kurihara;H. Wariishi;Hiroo Tanaka
利用差异显示反转录-聚合酶链式反应技术，研究了木质素降解担子菌黄孢原毛平革菌对外源二苯并对二恶英胁迫反应基因的表达。通过BLAST搜索，确定了6个与其他微生物蛋白质高度同源的基因片段：NADH-泛醌氧化还原酶(NUO)、ATP/ADP载体、尿酸-黄嘌呤渗透酶、锰超氧化物歧化酶、3-羟丁酰辅酶A脱氢酶和细胞骨架蛋白。邻苯二酚和三羟基苯也能上调Nuo的表达，但二苯并呋喃不能上调Nuo的表达，这表明Nuo的表达是由胞外单电子氧化酶与二苯并-对二恶英反应生成的苯二酚产物启动的。
The stress-responsive genes expressed against the exogenous addition of dibenzo-p-dioxin from the lignin-degrading basidiomycete, Phanerochaete chrysosporium, were determined utilizing a differential display reverse transcription-PCR technique. Six cDNA fragments, exhibiting a high homology with various proteins from other microorganisms, were identified via a BLAST search; that is, NADH-ubiquinone oxidoreductase (NUO), ATP/ADP carrier, uric acid-xanthine permease, manganese superoxide dismutase, 3-hydroxybutyryl-coenzyme A dehydrogenase, and cytoskeletal protein. The expression of NUO was also up-regulated by catechol and trihydroxybenzene but not by dibenzofuran, suggesting that NUO expression was initiated by the formation of quinone products through the reaction of extracellular one-electron oxidizing enzymes with dibenzo-p-dioxin.