A reversibly palmitoylated resident protein (p63) of an ER-Golgi intermediate compartment is related to a circulatory shock resuscitation protein.
A reversibly palmitoylated resident protein (p63) of an ER-Golgi intermediate compartment is related to a circulatory shock resuscitation protein.
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发表时间：
1993-03
影响因子：
4
通讯作者：
A. Schweizer;J. Rohrer;P. Jenö;A. DeMaio;T. Buchman;H. Hauri
中科院分区：
文献类型：
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作者：
A. Schweizer;J. Rohrer;P. Jenö;A. DeMaio;T. Buchman;H. Hauri
最近发现的63 kDa的膜蛋白，p63，是一个常驻蛋白的膜网络之间插入粗糙的ER和高尔基体。为了在分子水平上表征p63，已从人胎盘λ gt 10 cDNA文库中分离出编码p63的2.91 kb cDNA。从分离的p63制备的胰蛋白酶肽序列分析证实了克隆基因的鉴定。翻译的氨基酸序列由601个氨基酸（65.8kDa）组成，具有单个推定的跨膜区和106个氨基酸的N-末端胞质结构域。人p63 cDNA与猪肝cDNA 3AL（登录号M27092）具有高水平的序列同一性，后者的表达在循环休克复苏后增强。p63的另一个显著特征是，当细胞内蛋白质转运被药物布雷菲德菌素A阻断时，它会可逆地棕榈酰化。p63在COS细胞中的过表达导致细胞质中显著的管状膜网络的发展。这表明该蛋白可能是p63区室结构的决定因素。
The recently identified 63 kDa membrane protein, p63, is a resident protein of a membrane network interposed in between rough ER and Golgi apparatus. To characterize p63 at the molecular level a 2.91 kb cDNA encoding p63 has been isolated from a human placenta lambda gt10 cDNA library. Sequence analysis of tryptic peptides prepared from isolated p63 confirmed the identify of the cloned gene. The translated amino acid sequence consists of 601 amino acids (65.8 kDa) with a single putative membrane-spanning region and a N-terminal cytoplasmic domain of 106 amino acids. The human p63 cDNA exhibits a high level of sequence identify to the pig hepatic cDNA 3AL (accession number M27092) whose expression is enhanced after resuscitation from circulatory shock. An additional remarkable feature of p63 is that it becomes reversibly palmitoylated when intracellular protein transport is blocked by the drug brefeldin A. Overexpression of p63 in COS cells led to the development of a striking tubular membrane network in the cytoplasm. This suggests that the protein may be determinant for the structure of the p63 compartment.