Up-regulation of cyclic electron flow and down-regulation of linear electron flow in antisense-rca mutant rice
Up-regulation of cyclic electron flow and down-regulation of linear electron flow in antisense-rca mutant rice
复制标题
DOI：
10.1007/s11099-008-0086-9
复制
发表时间：
2008-12
期刊：
影响因子：
2.7
通讯作者：
Sheng Jin;Sheng Jin;Dun Wang;F.-Y. Zhu;X. Li;Jian Sun;D. Jiang
中科院分区：
文献类型：
--
作者：
Sheng Jin;Sheng Jin;Dun Wang;F.-Y. Zhu;X. Li;Jian Sun;D. Jiang
为了研究净光合速率(PN)仅为野生型亲本一半的核酮糖-1,5-双磷酸羧化酶/加氧酶激活酶反义转基因水稻中多余的激发能如何消散，我们测量了PN对细胞间CO2浓度(Ci)、电子传递速率(ETR)、光照下开放光系统2(PS2)反应中心的量子产率(Fv'/Fm')的响应曲线，总 PS2 中心效率 (ΦPS2)、光化学 (qP) 和非光化学猝灭 (NPQ)、辐照后叶绿素 (Chl) 荧光瞬时增加 (PITICF) 和 P700+ 再还原。在突变体中，羧化效率对Ci、饱和辐照度下的ETR以及辐照下的Fv'/Fm'、ΦPS2和qP的依赖性显着降低。然而，突变体中的 NPQ、能量依赖性猝灭 (qE)、PITICF 和 P700+ 再还原显着较高。因此，突变体下调线性 ETR 并刺激 PS1 周围的循环电子流，这可能会产生 ΔpH 以支持 NPQ 和 qE 消散多余的激发能量。
To investigate how excess excitation energy is dissipated in a ribulose-1,5-bisphospate carboxylase/oxygenase activase antisense transgenic rice with net photosynthetic rate (PN) half of that of wild type parent, we measured the response curve ofPNto intercellular CO2concentration (Ci), electron transport rate (ETR), quantum yield of open photosystem 2 (PS2) reaction centres under irradiation (Fv′/Fm′), efficiency of total PS2 centres (ΦPS2), photochemical (qP) and non-photochemical quenching (NPQ), post-irradiation transient increase in chlorophyll (Chl) fluorescence (PITICF), and P700+re-reduction. Carboxylation efficiency dependence onCi, ETR at saturation irradiance, and Fv′/Fm′, ΦPS2, and qPunder the irradiation were significantly lower in the mutant. However, NPQ, energy-dependent quenching (qE), PITICF, and P700+re-reduction were significantly higher in the mutant. Hence the mutant down-regulates linear ETR and stimulates cyclic electron flow around PS1, which may generate the ΔpH to support NPQ and qEfor dissipation of excess excitation energy.