The Electron Transport in psbS-Silenced Rice
The Electron Transport in psbS-Silenced Rice
复制标题
psbS-沉默稻中的电子传输
DOI：
10.1007/978-3-642-32034-7_101
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
T. Endo
中科院分区：
文献类型：
--
作者：
Ken;S. Ishida;K. Shimamoto;F. Sato;T. Endo
光系统II （PSII）吸收的一部分光能用于光合电子传递，其余部分以热和荧光的形式消散。虽然有一些方法可以估计叶绿素荧光对吸收光能的分配（例如，Dermming-Adamset al., 1996; Krameret al., 2004; Hendricksonet al., 2004），但没有一种方法得到实验的认可。为此，我们培育了psbS基因被有效沉默的抗psbS水稻株系。psbs基因的沉默导致PSII吸收光能分配到天线TD (D)的减少，在宽光强范围内PSII闭合中心TD (E)的吸收光能分配增加，而电子输运的能量分配(P)没有变化，说明psbs沉默线的主要耗散点从天线转移到PSII中心。这也意味着即使在弱光条件下，光合作用电子传递的限速步骤也不在光吸收处，而是在电子传递中的PSII受体侧。
A part of light energy absorbed in photosystem II (PSII) is used for photosynthetic electron transport and the rest is dissipated as heat and fluorescence. While there are methods to estimate the allocation of the absorbed light energy by chlorophyll fluorescence (for example, Dermming-Adamset al., 1996; Krameret al., 2004; Hendricksonet al., 2004), none of them have become experimentally approved. For this purpose we generated lines of psbS knockdown rice in which both of psbS genes were efficiently silenced.The silencing ofpsbSgenes resulted in a decrease in the allocation of the absorbed light energy in PSII to TD in antenna (D), and an increase in that to TD in closed PSII centers, designated as excess (E), in wide range of light intensities, whereas energy allocation to the electron transport (P) did not change, suggesting that the main site of dissipation was shifted from antenna to PSII centers in PsbS-silenced lines. This also means that the rate-limiting step of photosynthetic electron transport was not at the light absorption even under low light but at acceptor side of PSII in the electron transport.