A Preliminary Study of Biomonitoring for Bisphenol-A in Human Sweat.
A Preliminary Study of Biomonitoring for Bisphenol-A in Human Sweat.
复制标题
人体汗液中双酚 A 生物监测的初步研究。
DOI：
10.1093/jat/bkv055
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发表时间：
2015
影响因子：
2.5
通讯作者：
Stanford,JosephB
中科院分区：
文献类型：
--
作者：
Porucznik,ChristinaA;Cox,KyleyJ;Wilkins,DianaG;Anderson,DavidJ;Bailey,NicoleM;Szczotka,KathrynM;Stanford,JosephB
测量人体内分泌干扰物双酚A（BPA）的暴露受到大多数人普遍存在但短暂暴露的阻碍，加上短的代谢半衰期，导致个体间和个体内的差异很大。我们研究了在志愿者参与者中测量人体汗液中BPA多日暴露的可能性，目的是确定一种受时间变化影响较小的暴露评估方法。我们招募了50名参与者佩戴汗液收集贴片（PharmChek®）7天，同时收集每日第一次晨尿。使用我们先前验证的方法，用定量LC-MS-MS分析尿液和汗斑提取物。此外，一名人类志愿者每天食用一罐市售汤（16盎司，473立方厘米），持续3天，并收集尿液。在研究的3天内佩戴汗液贴片（n= 2，每组1个）。通过将BPA强化到汗液贴片制备的质量控制样本中检测到BPA，但仅在三个参与者贴片上检测到高于平均背景5倍的BPA。尽管对于故意暴露的个体，测得的最高尿BPA浓度为195 ng/mL，但在相应的汗液斑中未检测到高于背景的BPA。在这项初步调查中，使用主要佩戴在上臂外侧的汗片并没有检测到尿液监测记录的BPA暴露。汗液中不含BPA可能是由于几个因素，包括每个贴片的样本量不足，或自然汗液中BPA浓度极低等。
Measurement of human exposure to the endocrine disruptor bisphenol-A (BPA) is hampered by the ubiquitous but transient exposure for most individuals, coupled with a short metabolic half-life which leads to high inter- and intra-individual variability. We investigated the possibility of measuring multiday exposure to BPA in human sweat among volunteer participants with the goal of identifying an exposure assessment method less affected by temporal variability. We recruited 50 participants to wear a sweat collection patch (PharmChek®) for 7 days with concurrent collection of daily first-morning urine. Urines and sweat patch extracts were analyzed with quantitative LC–MS-MS using a method we previously validated. In addition, a human volunteer consumed one can of commercially available soup (16 oz, 473 cm3) daily for 3 days and collected urine. Sweat patches (n= 2, 1 per arm) were worn for the 3 days of the study. BPA was detected in quality control specimens prepared by fortification of BPA to sweat patches, but was only detected at 5× above average background on three participant patches. Although the highest measured urine BPA concentration was 195 ng/mL for an individual with deliberate exposure, no BPA was detected above background in the corresponding sweat patches. In this preliminary investigation, the use of sweat patches primarily worn on the upper-outer arm did not detect BPA exposures that were documented by urine monitoring. The absence of BPA in sweat patches may be due to several factors, including insufficient quantity of specimen per patch, or extremely low concentrations of BPA in naturally occurring sweat, among others.