Extremely low dose of acrylamide decreases lifespan in Caenorhabditis elegans
Extremely low dose of acrylamide decreases lifespan in Caenorhabditis elegans
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DOI：
10.1016/j.toxlet.2004.04.037
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发表时间：
2004-09-10
影响因子：
3.5
通讯作者：
Miwa, J
中科院分区：
文献类型：
--
作者：
Hasegawa, K;Miwa, S;Miwa, J
工业接触丙烯酰胺的神经毒性、遗传毒性和致癌作用已经在动物和人类身上进行了30多年的研究。最近一项对未接触工业的人体内丙烯酰胺本底含量高的原因的研究表明，丙烯酰胺是在油炸或烘烤食品时通过美拉德反应形成的。为了评估持续暴露于常见食品中含量的丙烯酰胺的生物学后果，我们研究了丙烯酰胺对秀丽隐杆线虫（Caenorhabditis elegans）三个参数的影响(1)生长，(2)繁殖和(3)寿命。在0.5 ~ 5mg /L浓度的丙烯酰胺环境下饲养，对动物的生长和繁殖力无不良影响。通常存在于日常食品中。相反，在500 mg/L的丙烯酰胺中，它们表现出生长迟缓，体和卵数减少。然而，与前两个参数不同的是，即使在0.5杯/升（世界卫生组织和美国环境保护局允许的饮用水中最大理论浓度）的丙烯酰胺中，寿命也会显著缩短。非常有趣的是，丙烯酰胺对寿命的影响是双相的，当浓度为5mg /L时，寿命增加到接近正常水平，而浓度为500mg /L时，寿命再次下降。2004爱思唯尔爱尔兰有限公司版权所有。
The neurotoxic, genotoxic and carcinogenic effects of industrial exposure to acrylamide have been studied in animals and humans for more than 30 years. A recent search for the cause of high background levels of acrylamide in industrially unexposed people revealed that it is formed during the frying or baking of foods by means of the Maillard reaction. To evaluate the biological consequences of continuous exposure to acrylamide at levels found in common foodstuffs, we studied the effects of acrylamide on the three parameters of (1) growth, (2) fecundity and (3) lifespan in the nematode Caenorhabditis elegans. As for growth and fecundity, no deleterious consequences were observed when the animals were raised in the acrylamide concentrations of 0.5 mug/L to 5 mg/L. which are commonly found in daily consumed foodstuffs. Conversely, in 500 mg/L of acrylamide, they showed retarded growth with reduced body and brood sizes. Unlike the first two parameters, however, lifespan decreased significantly even in 0.5 mug/L of acrylamide, the maximum theoretical concentration allowed in drinking water by the WHO and US EPA. Very interestingly, the acrylamide effect on lifespan is biphasic as lifespan increased to near normalcy in 5 mg/L and decreased again in 500 mg/L. (C) 2004 Elsevier Ireland Ltd. All rights reserved.