Identification of the S-transferase like superfamily bacillithiol transferases encoded by Bacillus subtilis.
Identification of the S-transferase like superfamily bacillithiol transferases encoded by Bacillus subtilis.
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DOI：
10.1371/journal.pone.0192977
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Pogliano K
中科院分区：
文献类型：
--
作者：
Perera VR;Lapek JD Jr;Newton GL;Gonzalez DJ;Pogliano K
杆菌硫醇是一种低分子硫醇，存在于细菌中，类似于谷胱甘肽，在这些细菌中是不存在的。杆菌硫醇转移酶催化杆菌硫醇转移到各种底物上。S类转移酶超家族包含30,000多个成员，其中包括杆状硫醇转移酶。这个家族中的蛋白质非常不同，通过结构而不是序列相似性来联系，这使得人们不清楚是否所有的蛋白质都具有相同的生化活性。枯草芽孢杆菌编码八个预测的STL超家族成员，其中只有一个被证明是杆状硫醇转移酶。在这里，我们发现剩下的七个蛋白质表现出不同水平的金属依赖杆状硫醇转移酶活性。我们已经将这八种酶重新命名为BSTA-H。质谱学和基因表达研究表明，所有这些酶在生长和产孢期都有不同程度的产生，其中BstB和BstE含量最高，BstF和BstH含量最低。有趣的是，在孢子形成过程中，母细胞中诱导了几种杆菌硫醇转移酶。缺乏所有八种杆菌硫醇转移酶的菌株在压力因素的存在下表现出正常的生长，这些压力因素对缺乏杆菌硫醇的菌株的生长产生不利影响，如百草枯和氯化镉。因此，STL杆状硫醇转移酶代表了一组新的蛋白质，它们在杆状硫醇依赖反应中发挥着目前未知但潜在的重要作用。我们的结论是，这些酶是高度不同的，可能是为了应对同样多样化的内源或外源有毒代谢物和氧化剂。
Bacillithiol is a low molecular weight thiol found in Firmicutes that is analogous to glutathione, which is absent in these bacteria. Bacillithiol transferases catalyze the transfer of bacillithiol to various substrates. The S-transferase-like (STL) superfamily contains over 30,000 putative members, including bacillithiol transferases. Proteins in this family are extremely divergent and are related by structural rather than sequence similarity, leaving it unclear if all share the same biochemical activity. Bacillus subtilis encodes eight predicted STL superfamily members, only one of which has been shown to be a bacillithiol transferase. Here we find that the seven remaining proteins show varying levels of metal dependent bacillithiol transferase activity. We have renamed the eight enzymes BstA-H. Mass spectrometry and gene expression studies revealed that all of the enzymes are produced to varying levels during growth and sporulation, with BstB and BstE being the most abundant and BstF and BstH being the least abundant. Interestingly, several bacillithiol transferases are induced in the mother cell during sporulation. A strain lacking all eight bacillithiol transferases showed normal growth in the presence of stressors that adversely affect growth of bacillithiol-deficient strains, such as paraquat and CdCl2. Thus, the STL bacillithiol transferases represent a new group of proteins that play currently unknown, but potentially significant roles in bacillithiol-dependent reactions. We conclude that these enzymes are highly divergent, perhaps to cope with an equally diverse array of endogenous or exogenous toxic metabolites and oxidants.
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影响因子：
--
作者：
Feng X;Hu Y;Zheng Y;Zhu W;Li K;Huang CH;Ko TP;Ren F;Chan HC;Nega M;Bogue S;López D;Kolter R;Götz F;Guo RT;Oldfield E
通讯作者：
Oldfield E
DOI：
10.1074/mcp.m113.034769
发表时间：
2014-01
期刊：
Molecular & cellular proteomics : MCP
影响因子：
--
作者：
Hebert AS;Richards AL;Bailey DJ;Ulbrich A;Coughlin EE;Westphall MS;Coon JJ
通讯作者：
Coon JJ
影响因子：
4.1
作者：
Fang, Zhong;Roberts, Alexandra A.;Dos Santos, Patricia C.
通讯作者：
Dos Santos, Patricia C.
影响因子：
48
作者：
Gibson, Daniel G.;Young, Lei;Smith, Hamilton O.
通讯作者：
Smith, Hamilton O.
影响因子：
3.5
作者：
GueroutFleury, AM;Frandsen, N;Stragier, P
通讯作者：
Stragier, P