Lipopolysaccharide-induced cytokine and receptor expression and neutrophil infiltration in the liver of osteopetrosis (op/op) mutant mice.
Lipopolysaccharide-induced cytokine and receptor expression and neutrophil infiltration in the liver of osteopetrosis (op/op) mutant mice.
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脂多糖诱导骨石症（op/op）突变小鼠肝脏中的细胞因子和受体表达以及中性粒细胞浸润。
DOI：
10.1034/j.1600-0676.2000.020006465.x
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
Shultz,LD
中科院分区：
文献类型：
--
作者：
Jiang,S;Naito,M;Kaizu,C;Kuwata,K;Hasegawa,G;Mukaida,N;Shultz,LD
背景/目的石骨症（op）突变纯合子小鼠在巨噬细胞集落刺激因子（M-CSF/CSF-1）中存在遗传缺陷，其特征在于巨噬细胞的分化和功能缺陷。本研究的目的是评估M-CSF的贡献脂多糖（LPS）诱导的细胞因子表达和中性粒细胞浸润在liver.MethodsWe调查的影响，LPS管理在M-CSF缺陷/op突变小鼠。通过免疫组织化学和RT-PCR研究肝脏中细胞因子和受体的表达。LPS给药后，细胞因子的产生和LPS受体的表达，如CD 14和清道夫受体A类（MSR-A），诱导在较低的水平inop/op小鼠比同窝小鼠。p/op小鼠肝脏中的神经元浸润与同窝小鼠无显著差异。抗IL-8受体同源物和抗C5 a受体抗体减少了浸润中性粒细胞的数量。结论这些研究结果表明，op/op小鼠注射LPS后巨噬细胞活化缺陷与肝脏中CD 14和MSR-A的表达减少有关。因此，M-CSF在LPS诱导的巨噬细胞活化中起关键作用，但在肝脏中性粒细胞浸润中不起主导作用。
Background/AimsMice homozygous for the osteopetrosis (op) mutation are genetically deficient in macrophage colony‐stimulating factor (M‐CSF/CSF‐1) and are characterized by defective differentiation and function of macrophages. The aim of this study is to assess the contribution of M‐CSF to lipopolysaccharide (LPS)‐induced cytokine expression and neutrophil infiltration in the liver.MethodsWe investigated the effects of LPS administration in M‐CSF‐deficientop/opmutant mice. The expression of cytokines and receptors in the liver was studied by immunohistochemistry and RT‐PCR. Neutrophil infiltration in the liver was also examined.ResultsAfter LPS administration, cytokine production and expression of LPS receptors, such as CD14 and scavenger receptor class A (MSR‐A), were induced at lower levels inop/opmice than those in littermate mice. Neutrophil infiltration in the liver ofop/opmice did not differ significantly from that of littermate mice. Anti‐IL‐8 receptor homologue and anti‐C5a receptor antibody reduced the number of infiltrating neutrophils.ConclusionsThese findings indicate that deficient macrophage activation following LPS injection inop/opmice is associated with decreased expression of CD14 and MSR‐A in the liver. Thus, M‐CSF plays a critical role in LPS‐induced macrophage activation but does not exert a dominant role in neutrophil infiltration in the liver.