LncRNA NONHSAT114552 Sponges miR-320d to Promote Proliferation and Invasion of Chordoma Through Upregulating NRP1.
LncRNA NONHSAT114552 Sponges miR-320d to Promote Proliferation and Invasion of Chordoma Through Upregulating NRP1.
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lncrna nonhsat114552 sponges miR-320d，通过上调NRP1促进核瘤的增殖和侵袭。
DOI：
10.3389/fphar.2021.773918
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发表时间：
2021
影响因子：
5.6
通讯作者：
Chen K
中科院分区：
文献类型：
--
作者：
Zhang K;Liu Z;Tang Y;Shao X;Hua X;Liu H;Yang H;Chen K
脊索瘤是一种较少见的恶性骨肿瘤，局部复发率高。到目前为止，这一机制仍不清楚。LncRNAs作为microRNAs的竞争性内源性RNAs，在肿瘤的发生发展中起着关键作用。然而，lncRNA在脊索瘤中的生物学作用尚不清楚。在本研究中，我们的目的是研究lncRNA NONHSAT114552在脊索瘤发生中的作用和调控机制。采用定量RT-PCR方法检测脊索瘤组织中NONHSAT114552和miR-320d的表达水平。同时还探讨了NONHSAT114552与临床预后的关系。通过生物信息学分析和荧光素酶报告基因分析，验证了NONHSAT114552与miR-320d、miR-320d与Neuropilin 1(Nrp1)之间的关系。此外，还观察了NONHSAT114552对脊索瘤细胞(U-CH1和U-CH2)增殖和侵袭的影响及其对miR-320d的调节作用。此外，还研究了NONHSAT114552/miR-320d/Nrp1轴在体内对脊索瘤发生的影响。与胎儿髓核相比，脊索瘤组织中NONHSAT114552表达上调，miR-320d表达下调。Kaplan-Meier生存分析显示，NONHSAT114552过表达与患者预后不良有关。NONHSAT114552基因的敲除可显著抑制脊索瘤细胞的增殖和侵袭。体外研究证实，NONHSAT114552通过直接海绵结合miR-320d来调控Nrp1，从而促进脊索瘤细胞的增殖和侵袭。体内研究表明，NONHSAT114552通过海绵miR-320d调节Nrp1抑制脊索瘤的生长。我们的研究结果表明，lncRNA NONHSAT114552在脊索瘤的发生发展中起着重要作用，它可能成为脊索瘤的一个潜在的预后标志物和治疗靶点。。
Chordoma is a relatively rare malignant bone tumor with high local recurrence. To date, the mechanism remains unclear. lncRNAs play a pivotal role in tumorigenesis by acting as competitive endogenous RNAs of microRNAs. However, the biological role of lncRNA is still unclear in chordoma. In this research, our aim is to investigate the roles and regulation mechanisms of lncRNA NONHSAT114552 in chordoma development. The expression level of NONHSAT114552 and miR-320d in chordoma tissues was determined by qRT-PCR. Meantime, the correlation between NONHSAT114552 and clinical prognosis was also studied. Bioinformatics analysis and luciferase reporter assays were used to verify the relationship between NONHSAT114552 and miR-320d, and between miR-320d and Neuropilin 1 (NRP1). In addition, effects of NONHSAT114552 on chordoma cells (U-CH1 and U-CH2) proliferation and invasion and its regulation on miR-320d were also evaluated. Furthermore, the influences of NONHSAT114552/miR-320d/NRP1 axis on chordoma tumorigenesis were investigated in vivo. NONHSAT114552 was overexpressed while miR-320d was down-regulated in chordoma tissue compared to fetal nucleus pulposus. Kaplan-Meier survival analysis showed that NONHSAT114552 overexpression was associated with patients’ poor prognosis. Knockdown of NONHSAT114552 significantly suppressed chordoma cell proliferation and invasion. In vitro studies confirmed that NONHSAT114552 acted as ceRNA to regulate NRP1 by directly sponging miR-320d, thus facilitating chordoma cell proliferation and invasion. In vivo study demonstrated that NONHSAT114552 moderated chordoma growth by sponging miR-320d to regulating NRP1. Our findings indicate that lncRNA NONHSAT114552 exhibits a critical role in the tumorigenesis and development of chordoma and it may become one potential prognostic marker and therapeutic target for this disease. .
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影响因子：
9
作者：
Zhang H;Yang K;Ren T;Huang Y;Tang X;Guo W
通讯作者：
Guo W
影响因子：
2.6
作者：
Lu, Lin;Zhang, Lei;Han, Zhong Chao
通讯作者：
Han, Zhong Chao
影响因子：
25.7
作者：
Berge, Mathieu;Allanic, David;Merkulova-Rainon, Tatyana
通讯作者：
Merkulova-Rainon, Tatyana
影响因子：
2.8
作者：
Ruosi, C.;Colella, G.;Fazioli, F.
通讯作者：
Fazioli, F.
DOI：
10.26355/eurrev_202002_20151
发表时间：
2020-01-01
影响因子：
3.3
作者：
Wang, C-B;Wang, Y.;Guo, X-L
通讯作者：
Guo, X-L