DETECTION OF IMMUNOGLOBULIN-M ANTIBODY TO EPSTEIN-BARR VIRUS BY USE OF AN ENZYME-LABELED ANTIGEN
DETECTION OF IMMUNOGLOBULIN-M ANTIBODY TO EPSTEIN-BARR VIRUS BY USE OF AN ENZYME-LABELED ANTIGEN
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DOI：
10.1128/jcm.16.2.361-366.1982
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发表时间：
1982-01-01
影响因子：
9.4
通讯作者：
SCHMITZ, H
中科院分区：
文献类型：
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作者：
SCHMITZ, H
通过使用包被有抗-μ-IgM抗体的微量滴定板检测针对爱泼斯坦-巴尔病毒的IgM抗体。链抗血清和酶标EB病毒抗原。确定了标记的最佳条件。向酶标抗原中加入未标记的对照抗原可有效减少背景反应。修辞因素不再干扰。也不再观察到IgG对特异性IgM抗体的阻断。比较了四种不同的检测急性EB病毒感染的方法。酶标抗原-IgM试验与EB核抗原或嗜异性抗原抗体的检测相结合具有高度的敏感性和特异性。通过改变固相，可以检测到EB病毒的伊加抗体。
IgM antibodies to Epstein-Barr virus were detected by using microtiter plates coated with anti-.mu.-chain antiserum and enzyme-labeled Epstein-Barr virus antigen. Optimum conditions for labeling were determined. The addition of unlabeled control antigen to the enzyme-labeled antigen was effective in reducing background reactions. Rheumatoid factor no longer interfered. Blocking of specific IgM antibody by IgG also was no longer observed. Four different methods for the detection of acute Epstein-Barr virus infections were compared. A combination of the enzyme-labeled antigen-IgM test with the detection of antibodies to either Epstein-Barr nuclear antigen or heterophile antigen was highly sensitive and specific. By changing the solid phase, IgA antibodies to Epstein-Barr virus could be detected.