Genomic imbalances in human lung adenocarcinomas and squamous cell carcinomas
Genomic imbalances in human lung adenocarcinomas and squamous cell carcinomas
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DOI：
10.1002/gcc.1145
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发表时间：
2001-07-01
影响因子：
3.7
通讯作者：
Testa, JR
中科院分区：
文献类型：
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作者：
Pei, JM;Balsara, BR;Testa, JR
对67例非小细胞肺癌(NSCLC)，包括32例鳞癌(SCCs)和35例腺癌(ACS)进行比较基因组杂交分析，以确定这两种组织亚型之间基因组失衡模式的差异。在所有的肿瘤中。最常见的过度表达的染色体臂是1q、3q、5p和8q，每个都在50%-55%的病例中被检测到。最常被低估的手臂是9q、3p、8p和17p。鳞状细胞癌和急性冠脉综合征的失衡数量相似(中位数分别为12例和11例)。此外，许多不平衡，如1q、5p和8q的收益，在两个组织学亚组中都出现了很高的频率。然而，发现了几个统计上显著的差异。最显著的差异是3q24-QTER扩增，81%的鳞癌与31%的急性冠状细胞癌(P<；0.0001)相比，在32例鳞癌中有8例(25%)有3q25-26的扩增，而在35例急性冠状细胞癌中只有2例(6%)有3q25-26的扩增。鳞状细胞癌中20p13的增加和4q的丢失的发生率也显著高于急性冠脉综合征，而6p的过度表达在急性冠脉综合征中更为常见。7q和8q的增加分别与较高的肿瘤分期和阳性淋巴结转移或较高的肿瘤分级有关。这些数据表明，位于几个染色体区域的基因，特别是3q25-26，可能与区分肺鳞状细胞癌和急性冠脉综合征的表型特征有关。此外，某些失衡，特别是7q和8q的增益，可能预示着非小细胞肺癌的肿瘤侵袭性。(C)2001年Willey-Liss公司
Comparative genomic hybridization analysis was performed on 67 non-small-cell lung cancers (NSCLCs), including 32 squamous cell carcinomas (SCCs) and 35 adenocarcinomas (ACs), to identify differences in the patterns of genomic imbalance between these two histologic subtypes. Among the entire tumor set. the chromosome arms most often overrepresented were 1q, 3q, 5p, and 8q, each detected in 50-55% of cases. The most frequently underrepresented arms were 9q, 3p, 8p, and 17p. The number of imbalances was similar in SCCs and ACs (median number/case: 12 and 11, respectively). Moreover, many imbalances, such as gains of 1q, 5p, and 8q, occurred at a high frequency in both histologic subgroups. Several statistically significant differences, however, were found. The mon prominent difference was gain of 3q24-qter, seen in 81% of SCCs compared with 31% of ACs (P < 0.0001), with amplification at 3q25-26 being detected in eight of 32 (25%) SCCs but in only two of 35 (6%) ACs. Gain of 20p13 and loss of 4q also were seen at a significantly higher rate in SCCs than in ACs, whereas overrepresentation of 6p was more common in ACs. Gains of 7q and 8q each were associated with higher-stage tumors and either positive nodal involvement or higher tumor grade. These data suggest that genes located in several chromosomal regions, particularly 3q25-26, may be associated with phenotypic properties that differentiate lung SCCs from ACs. Furthermore, certain imbalances, prominent among them gains of 7q and 8q, may be indicative of tumor aggressiveness in NSCLCs. (C) 2001 Willey-Liss, Inc.