Synthesis and characterization of hyaluronic acid/human-like collagen hydrogels
Synthesis and characterization of hyaluronic acid/human-like collagen hydrogels
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透明质酸/类人胶原水凝胶的合成与表征
DOI：
10.1016/j.msec.2014.07.058
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发表时间：
2014-10-01
影响因子：
7.9
通讯作者：
Ma, Pei
中科院分区：
文献类型：
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作者：
Zhang, Jingjing;Ma, Xiaoxuan;Ma, Pei
可注射水凝胶在软组织填充和修复中起着重要的作用。我们报道了一种基于透明质酸（HA）和类人胶原蛋白（HLC）的可注射水凝胶，两者都具有良好的生物相容性和可生物降解性。这两类生物大分子与1,4-丁二醇二甘油酯醚交联形成三维网络。通过透析和蒸馏去除多余的交联剂。以相同方法制备的ha基水凝胶作为对照。细胞相容性采用细胞计数试剂盒-8 （CCK-8）检测。采用咔唑比色法测定其体外降解率。采用苏木精和伊红（H&E）染色和免疫组织化学分析评价组织相容性。CCK-8实验表明，HA/ hplc水凝胶比HA基水凝胶具有更小的细胞毒性，并能促进幼鼠肾细胞（BHK）的增殖。细胞黏附表明BHK能在材料表面生长良好，并保持良好的细胞活力。体外降解实验表明，HA/ hplc水凝胶具有较长的降解时间和较好的抗酶能力。体内注射显示，1、2、4周后，HA/HLC几乎没有炎症反应。因此，透明质酸/高效液相色谱水凝胶是一种很有前途的软组织填充和修复材料。(C) 2014 Elsevier B.V.版权所有
Injectable hydrogel plays an important role in soft tissue filling and repair. We report an injectable hydrogel based on hyaluronic acid (HA) and human-like collagen (HLC), both with favorable biocompatibility and biodegradability. These two types of biomacromolecules were crosslinked with 1,4-butanediol diglycidyl ether to form a three-dimensional network. The redundant crosslinker was removed by dialysis and distillation. An HA-based hydrogel prepared by the same method was used as a control. The cytocompatibility was studied with a Cell Counting Kit-8 (CCK-8) test. Carbazole colorimetry was used to analyze the in vitro degradation rate. The histocompatibility was evaluated by hematoxylin and eosin (H&E) staining analysis and immunohistochemical analysis. The CCK-8 assay demonstrated that the HA/HLC hydrogel was less cytotoxic than the HA-based hydrogel and could promote baby hamster kidney cell (BHK) proliferation. The cell adhesion indicated that BHK could grow well on the surface of the materials and maintain good cell viability. The in vitro degradation test showed that the HA/HLC hydrogel had a longer degradation time and an excellent antienzyme ability. In vivo injection showed that there was little inflammatory response to HA/HLC after 1, 2, and 4 weeks. Therefore, the HA/HLC hydrogel is a promising biomaterial for soft tissue filling and repair. (C) 2014 Elsevier B.V. All rights reserved.