Stimuli affecting zebrafish (Danio rerio) behavior in the light/dark preference test
Stimuli affecting zebrafish (Danio rerio) behavior in the light/dark preference test
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DOI：
10.1016/j.physbeh.2011.07.029
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发表时间：
2011-10-24
影响因子：
2.9
通讯作者：
Penalosa, Y. M.
中科院分区：
文献类型：
--
作者：
Blaser, R. E.;Penalosa, Y. M.
乙醇被认为对斑马鱼有抗焦虑作用，主要是基于它破坏了斑马鱼的新型潜水反应和一些社会行为。光/暗偏好测试为鱼类焦虑样行为提供了一种补充测量方法，当前研究的目的是确定急性乙醇暴露对光/暗任务行为的影响。在实验1中，为了确定如何最好地测量斑马鱼的行为，我们使用了不同的刺激来诱导斑马鱼的光/暗偏好。当光照被操纵时，受试者表现出趋光性（对光的偏好），而当墙壁和基底颜色被操纵时，受试者表现出趋光性（对暗的偏好）。运动活动和颜色偏好之间存在明显的相互作用，动物倾向于在较暗的地方冻结。由于开放/覆盖版本的测试在解释行为上存在歧义，实验2采用黑白版本。在实验2。将斑马鱼暴露于乙醇（0.25%）中。0.5%。或1.0%)或水浸泡30分钟，然后放入含有乙醇（相同剂量）或水的黑白偏好箱中进行30分钟的测试。乙醇暴露增加了运动活动，减少了冻结。此外，乙醇处理与侧位偏好的运动活动之间存在显著的相互作用。低剂量的乙醇增加了正常游动的鱼对白色的回避，而高剂量则没有。(C) 2011爱思唯尔公司版权所有。
Ethanol has been suggested to have an anxiolytic effect on zebrafish, primarily based on its disruption of the novel tank diving response and of some social behaviors. The light/dark preference test offers a complementary measure of anxiety-like behavior in fish, and the purpose of the current study was to determine the effects of acute ethanol exposure on behavior in the light/dark task. In Experiment 1, the stimuli used to induce light/dark preference in zebrafish were varied in order to determine how best to measure the behavior. Subjects exhibited phototaxis (preference for light) when illumination was manipulated, but scototaxis (preference for dark) when wall and substrate color were manipulated. There was a clear interaction between locomotor activity and color preference, with animals preferentially freezing in darker locations. Because of ambiguity in interpreting behavior in the open/covered version of the test, the black/white version was used in Experiment 2. In Experiment 2. zebrafish were exposed to ethanol (0.25%. 0.5%. or 1.0%) or water for 30 minutes, and then placed in a black/white preference tank containing either ethanol (same doses) or water for a 30-minute test. Ethanol exposure increased locomotor activity and reduced freezing. Additionally, there was a significant interaction between ethanol treatment and locomotor activity on side preference. Low doses of ethanol increased white avoidance in normally swimming fish, while high doses did not. (C) 2011 Elsevier Inc. All rights reserved.