Increased amplification of pBR322 plasmid deoxyribonucleic acid in Escherichia coli K-12 strains RR1 and chi1776 grown in the presence of high concentrations of nucleoside
Increased amplification of pBR322 plasmid deoxyribonucleic acid in Escherichia coli K-12 strains RR1 and chi1776 grown in the presence of high concentrations of nucleoside
复制标题
在高浓度核苷存在下生长的大肠杆菌 K-12 菌株 RR1 和 chi1776 中 pBR322 质粒脱氧核糖核酸的扩增增加
DOI：
10.1128/jb.138.1.270-272.1979
复制
发表时间：
1979
影响因子：
3.2
通讯作者：
J. Monahan
中科院分区：
文献类型：
--
作者：
M. Norgard;Kirsten Emigholz;J. Monahan
当携带pBR 322质粒的大肠杆菌菌株RR 1或chi1776在每毫升含1毫克尿苷或胞苷的条件下生长，然后用氯霉素处理时，回收的质粒脱氧核糖核酸比常规质粒扩增方法通常获得的多三倍。
When pBR322 plasmid-harboring Escherichia coli strains RR1 or chi1776 were grown in the presence of 1 mg of uridine or cytidine per ml and later treated with chloramphenicol, as much as three times more plasmid deoxyribonucleic acid was recovered than would normally be obtained by routine plasmid amplification procedures.