Neuroprotective iridoid glycosides from Cornus officinalis fruits against glutamate-induced toxicity in HT22 hippocampal cells
Neuroprotective iridoid glycosides from Cornus officinalis fruits against glutamate-induced toxicity in HT22 hippocampal cells
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DOI：
10.1016/j.phymed.2011.08.068
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发表时间：
2012-02-15
期刊：
影响因子：
7.9
通讯作者：
Kim, Young Choong
中科院分区：
文献类型：
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作者：
Jeong, Eun Ju;Kim, Tae Bum;Kim, Young Choong
本文报道了Comas officinalis S et Z果实的甲醇提取物。（山茱萸科）对HT 22海马细胞谷氨酸诱导的毒性表现出显著的神经保护活性。本文研究了川楝子甲醇提取物的正丁醇组分的化学组成。采用高效液相色谱-二极管阵列检测器-电喷雾-质谱（HPLC-DAD-ESI-MS）技术，筛选出活性最强的一个化合物。从正丁醇部位分离得到5个环烯醚萜苷类化合物，其中包括1个新化合物：7-O-丁基莫诺苷（1）、马钱苷（2）、莫诺苷（3）、7 R-O-甲基莫诺苷（4）、7S-O-甲基莫诺苷（5）。分离的化合物本身的保护活性在统计学上不显著。然而，化合物1、4和5的水解产物显著保护谷氨酸损伤的HT 22细胞，分别高达未处理对照的78 +/-2.2%，60 +/-3.2%和59 +/-2.5%。(C)2011年Elsevier GmbH。All rights reserved.
The methanolic extract of the fruits of Comas officinalis S et Z. (Cornaceae) showed the significant neuroprotective activity against glutamate-induced toxicity in HT22 hippocampal cells. Chemical profile of n-BuOH fraction of the methanolic extract of C. officinalis fruits, which showed the most potent activity, was established using HPLC-diode array detector-electrospray-MS (HPLC-DAD-ESI-MS). Through bioactivity-guided isolation, five iridoid glycosides including one new compound, 7-O-butylmorroniside (1), loganin (2), morroniside (3), 7R-O-methylmorroniside (4), 7S-O-methylmorroniside (5) were isolated from the n-BuOH fraction. The protective activities of the isolated compounds, themselves, were not statistically significant. However, the hydrolyzed products of compounds 1,4 and 5 significantly protected glutamate-injured HT22 cells up to 78 +/- 2.2%, 60 +/- 3.2% and 59 +/- 2.5% of non-treated control, respectively. (C) 2011 Elsevier GmbH. All rights reserved.