Immunohistochemical analysis of Metadherin in proliferative and cancerous breast tissue.
Immunohistochemical analysis of Metadherin in proliferative and cancerous breast tissue.
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DOI：
10.1186/1746-1596-5-38
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发表时间：
2010-06-18
影响因子：
2.6
通讯作者：
Yang Q
中科院分区：
文献类型：
--
作者：
Su P;Zhang Q;Yang Q
据报道，金属粘附素(MTDH)与包括乳腺癌在内的各种人类癌症的进展有关。亚甲基四氢叶酸脱氢酶是否与乳腺癌的发生有关尚不清楚。本研究通过检测正常乳腺组织、导管非典型增生(ADH)、导管原位癌(DCIS)和浸润性癌组织中MTDH的表达，探讨其在乳腺癌发生发展中的可能作用。对手术切除的乳腺标本石蜡切片进行免疫组织化学染色。免疫组织化学结果显示正常组织几乎不表达，ADH和UDH呈中等表达，DCIS和癌组织呈MTDH强表达。统计学分析显示，MTDH在乳腺增生性病变和癌性病变中的表达有显著差异(p<；0.001)。癌组织分化程度与MTDH值呈正相关(p=0.028)。在乳腺癌中，统计分析显示mtdh的表达与患者的年龄(p=0.042)、ER状态(p=0.018)和p53状态(p=0.001)显著相关。我们还检测了mtdh对DCIS和癌细胞增殖的影响，发现mtdh过表达与高Ki67指数显著相关(分别为p=0.008和p=0.036)。在增生性乳腺病变中可以发现MTDH的过度表达，并可能与乳腺癌的进展有关。
Metadherin (MTDH) has been reported to be associated with cancer progression in various types of human cancers including breast cancer. Whether MTDH contributes to carcinogenesis of breast cancer is still unknown. In the present study, we investigated the expression of MTDH in normal, UDH (usual ductal hyperplasia), ADH (atypical ductal hyperplasia), DCIS (ductal carcinoma in situ) and invasive cancer to explore the possible role of MTDH for breast cancer carcinogenesis. Immunohistochemistry was employed on paraffin sections of surgical removed breast samples. The immunohistochemical results showed almost no staining in normal tissue, moderate staining in ADH and UDH, intense MTDH stains in DCIS and cancer. Statistical analysis demonstrated significant different MTDH expression between proliferative and cancerous breast lesions (p < 0.001). MTDH was positively correlated with the histological differentiation of DCIS (p = 0.028). In breast cancer, statistical analysis revealed a significant correlation between MTDH expression with patients' age (p = 0.042), ER status (p = 0.018) and p53 status (p = 0.001). We also examined the effect of MTDH on cell proliferation in DCIS and cancer, and we found that MTDH overexpression was significantly correlated with high Ki67 index (p = 0.008 and p = 0.036, respectively). MTDH overexpression could be identified in proliferative breast lesions and may contribute to breast cancer progression.
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影响因子：
50.3
作者：
Hu G;Chong RA;Yang Q;Wei Y;Blanco MA;Li F;Reiss M;Au JL;Haffty BG;Kang Y
通讯作者：
Kang Y
星形胶质细胞升高基因 1 通过抑制转录因子 FOXO1 促进乳腺癌增殖
影响因子：
8
作者：
Li, J.;Yang, L.;Li, M.
通讯作者：
Li, M.
AEG-1 的过度表达与人类非小细胞肺癌的肿瘤侵袭性和不良预后显着相关
影响因子：
7.3
作者：
Song, Libing;Li, Wen;Li, Jun
通讯作者：
Li, Jun
DOI：
10.1158/1078-0432.ccr-09-0049
发表时间：
2009-09-15
期刊：
Clinical cancer research : an official journal of the American Association for Cancer Research
影响因子：
--
作者：
Hu G;Wei Y;Kang Y
通讯作者：
Kang Y
影响因子：
8
作者：
Su, ZZ;Kang, DC;Fisher, PB
通讯作者：
Fisher, PB