Saccharomyces cerevisiae Bor1p is a boron exporter and a key determinant of boron tolerance.
Saccharomyces cerevisiae Bor1p is a boron exporter and a key determinant of boron tolerance.
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DOI：
10.1111/j.1574-6968.2006.00556.x
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发表时间：
2007-02
影响因子：
2.1
通讯作者：
Junpei Takano;Masaharuo Kobayashi;Y. Noda;T. Fujiwara
中科院分区：
文献类型：
--
作者：
Junpei Takano;Masaharuo Kobayashi;Y. Noda;T. Fujiwara
硼过量时对生物体是有毒的。酿酒酵母（Saccharomyces cerevisiae） Bor1p是一种质膜蛋白，可降低细胞内硼的浓度，使酵母具有硼耐受性。研究了硼1p转运硼的详细特征及其在硼耐受性中的作用。硼转运试验表明，bor1缺失突变体（bor1Delta）在细胞内积累了较高的硼浓度，并具有较低的硼输出速率。与野生型菌株相比，bor1Delta菌株对高浓度硼表现出更强的敏感性，其生长速率与细胞内硼浓度呈负相关。在正常到有毒的外部硼水平下，无论培养基中硼的浓度如何，绿色荧光蛋白（GFP）标记的Bor1p都定位在质膜上。综上所述，这些结果表明Bor1p是质膜硼输出者，也是硼耐受性的关键决定因素。
Boron is toxic to living organisms when present in excess. Saccharomyces cerevisiae Bor1p is a plasma membrane protein that decreases the intracellular concentration of boron and confers boron tolerance in yeasts. We investigated the detailed characteristics of boron transport by Bor1p and its roles in boron tolerance. Boron transport assays showed that the bor1 deletion mutant (bor1Delta) accumulates higher intracellular concentrations of boron and has a lower rate of boron export. The bor1Delta showed greater susceptibility to high concentrations of boron than the wild-type strain, and the growth rates of both strains were negatively correlated with the intracellular concentrations of boron. With normal to toxic levels of external boron, green fluorescent protein (GFP)-tagged Bor1p localized to the plasma membrane irrespective of the concentration of boron in the medium. Taken together, these results establish Bor1p as a plasma membrane boron exporter and a key determinant of boron tolerance.