Uniform-size hydrophobic polymer-based separation media selectively modified with a hydrophilic external polymeric layer.
Uniform-size hydrophobic polymer-based separation media selectively modified with a hydrophilic external polymeric layer.
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采用亲水性外部聚合物层选择性改性的均匀尺寸疏水性聚合物基分离介质。
DOI：
10.1016/0021-9673(94)00899-k
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Fréchet,JM
中科院分区：
文献类型：
--
作者：
Hosoya,K;Kishii,Y;Kimata,K;Araki,T;Tanaka,N;Svec,F;Fréchet,JM
提出了一种制备具有亲水外表面的大孔疏水苯乙烯-二乙烯基苯聚合物分离介质的简单方法。在制备尺寸-单分散颗粒的最后聚合步骤中，在反应混合物中加入亲水性单体和水溶性聚合引发剂。由于珠粒的疏水性苯乙烯-二乙烯基苯骨架已经形成，亲水单体没有穿透充满疏水性孔隙素的珠粒的孔隙，因此亲水层仅在珠粒的表面形成。所使用的亲水性单体包括单甲基丙烯酸甘油和二甲基丙烯酸甘油，并以甲苯作为聚苯乙烯-二乙烯基苯珠的孔隙剂。在有亲水性介质或没有亲水性介质的情况下进行的比较实验表明，介质对疏水溶质的分离选择性保持不变。然而，具有亲水性层的改性介质可以用于分析含有大肽分子的混合物，因为这些分子不会像未经改性的疏水珠那样在其表面吸附。
A simple procedure for the preparation of macroporous hydrophobic styrene-divinylbenzene polymeric separation media with a hydrophilic outer surface has been developed. A hydrophilic monomer and water-soluble polymerization initiator are added to the reaction mixture during the final polymerization step of the preparation of size-monodisperse particles. Because the hydrophobic styrene-divinylbenzene framework of the beads is already formed, and the hydrophilic monomer does not penetrate the pores of the beads that are filled with a hydrophobic porogen, the hydrophilic layer is formed only at the surface of the beads. The hydrophilic monomers used included glycerol monomethacrylate and glycerol dimethacrylate and toluene was used as the porogen for the poly(styrene-co-divinylbenzene) beads. Comparative experiments involving beads with or without a hydrophilic medium showed that the separation selectivity of the media towards hydrophobic solutes remains unchanged. However, the modified medium with a hydrophilic layer could be used to analyse mixtures that also contained large peptide molecules as these do not adsorb at its surface as is the case with the unmodified hydrophobic beads.