Diagnostic utility of anti-Ro52 detection in systemic autoimmunity
Diagnostic utility of anti-Ro52 detection in systemic autoimmunity
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DOI：
10.1136/pgmj.2009.089656
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发表时间：
2010-02-01
影响因子：
5.1
通讯作者：
Roberts-Thomson, Peter John
中科院分区：
文献类型：
--
作者：
Dugar, Manish;Cox, Sally;Roberts-Thomson, Peter John
目的确定单特异性抗Ro 52抗体的流行率和诊断价值，（定义为对Ro-52抗原的免疫应答，而对Ro-60抗原没有反应性）在选定的自身免疫性疾病中的反应性。研究设计储存从患有系统性红斑狼疮（SLE）、原发性干燥综合征（pSS）、系统性硬化症、结果402例标本中51例（12.7%）抗Ro 52抗体呈单特异性反应。抗Ro 52抗体是IIM患者中最常见的血清学标志物（35/147，23.8%），与抗Jo 1抗体同时出现（10/18，55.6%; p = 0.02）。在IIM、系统性硬化症、原发性胆汁性肝硬化、混合型原发性冷球蛋白血症和pSS患者中，抗Ro 52反应性的患病率显著高于抗Ro 60反应性。抗Ro 52反应的平均信号强度在pSS中显著高于SLE，并且与类风湿因子阳性相关。结论抗Ro 52单特异性抗体反应性不具有疾病特异性，但在IIM患者中可能具有重要意义。此外，由于抗Ro 52反应性在某些自身免疫性疾病中比抗Ro 60反应性更普遍，因此建议在临床实践中进行特异性检测以区分它们。
Objectives To determine the prevalence and diagnostic utility of monospecific anti-Ro52 (defined as an immune response against Ro-52 antigen in the absence of reactivity to Ro-60 antigen) reactivity in selected autoimmune diseases.Study design Stored diagnostic non-consecutive serum samples obtained from patients with systemic lupus erythematosus (SLE), primary Sjogren's syndrome (pSS), systemic sclerosis, idiopathic inflammatory myopathies (IIM), rheumatoid arthritis, primary biliary cirrhosis and mixed essential cryoglobulinaemia were analysed by line immunoassay to detect the presence of anti-Ro52 and other autoantibodies.Results Monospecific anti-Ro52 reactivity was found in 51 (12.7%) of the 402 samples tested. Anti-Ro52 was the most common serological marker in patients with IIM (35/147, 23.8%) and co-occurred with anti-Jo1 (10/18, 55.6%; p = 0.02). The prevalence of anti-Ro52 reactivity was significantly more than anti-Ro60 reactivity in patients with IIM, systemic sclerosis, primary biliary cirrhosis, mixed essential cryoglobulinemia and pSS. The mean signal intensity of anti-Ro52 reactivity was significantly higher in pSS than SLE and associated with rheumatoid factor positivity. The mean signal intensity of anti-Ro52 correlated with anti-Ro60 and anti-La in pSS and SLE.Conclusions Monospecific anti-Ro52 reactivity is not disease specific but may be of importance in patients with IIM. Furthermore, as anti-Ro52 reactivity is more prevalent than anti-Ro60 reactivity in certain autoimmune conditions, specific testing for their distinction in clinical practice is recommended.