Mechanical stimulation of gingiva reduces plasma 8-OHdG level in rat periodontitis
Mechanical stimulation of gingiva reduces plasma 8-OHdG level in rat periodontitis
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DOI：
10.1016/j.archoralbio.2007.10.005
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发表时间：
2008-04-01
影响因子：
3
通讯作者：
Watanabe, Tatsuo
中科院分区：
文献类型：
--
作者：
Ekuni, Daisuke;Tomofuji, Takaaki;Watanabe, Tatsuo
目的：牙龈细胞对牙周病原体的反应是通过产生活性氧来实现的，这种状态会增加循环中的氧化应激。通过刷牙改善牙龈炎症，不仅对牙周健康有临床益处，而且对循环状况也有好处。采用大鼠牙周炎模型，观察机械刺激对大鼠血浆8-羟基脱氧鸟苷水平的影响。设计：雄性Wistar大鼠18只，随机分为3组。对照组用无热原水局部涂抹牙龈沟，另两组局部涂抹细菌病原体(脂多糖和蛋白酶)，疗程8周。4周后，实验组中有一半的大鼠每天接受电动牙刷机械刺激4周。结果：细菌致病菌处理的大鼠出现牙周组织损伤，血浆8-羟基脱氧鸟苷水平升高。刷牙机械刺激可降低牙周组织中8-羟基脱氧鸟苷的含量，从而减少牙周炎和DNA氧化损伤。结论：牙周病患者牙周组织中的8-羟基脱氧鸟苷水平降低，可能与牙周循环中氧化应激相关分子的减少有关。(C)2007爱思唯尔有限公司。保留所有权利。
Objective: Gingival cells respond to periodontal pathogens by generating reactive oxygen species, and such a condition would increase circulating oxidative stress. Improvement of gingival inflammation by toothbrushing may offer clinical benefits on not only periodontal health but also the circulatory conditions. We examined the effects of mechanical stimulation on the plasma 8-hydroxydeoxyguanosine level in a rat periodontitis model.Design: In this experiment, male Wistar rats (n = 18) were divided into three groups. The control group received topical application of pyrogen-free water to the gingival sulcus for 8 weeks, while the other two groups received topical application of bacterial pathogens (lipopolysaccharide and proteases). After 4 weeks, half of the rats in the experimental groups received daily mechanical stimulation with an electric toothbrush for 4 weeks.Results: Rats treated with bacterial pathogens presented periodontal tissue damage and increased plasma levels of 8-hydroxydeoxyguanosine. Mechanical stimulation by toothbrushing decreased gingival inflammation and oxidative DNA damage indicated by a decrease in plasma 8-hydroxydeoxyguanosine.Conclusions: Mechanical stimulation of periodontally involved gingiva reduced 8-hydroxydeoxyguanosine in plasma and may contribute to a reduction in circulating oxidative stress associated molecules. (C) 2007 Elsevier Ltd. All rights reserved.