A novel kinase subverts aluminium resistance by boosting ornithine decarboxylase‐dependent putrescine biosynthesis
A novel kinase subverts aluminium resistance by boosting ornithine decarboxylase‐dependent putrescine biosynthesis
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一种新型激酶通过促进鸟氨酸脱羧酶依赖性腐胺生物合成来颠覆铝抗性
DOI：
10.1111/pce.14371
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Zheng Shao J.
中科院分区：
文献类型：
--
作者：
Liu Xiang P.;Gao Li J.;She Ben T.;Li Gui X.;Wu Yun R.;Xu Ji M.;Ding Zhong J.;Ma Jian F.;Zheng Shao J.
水稻作为最耐铝的谷类作物之一，其耐铝机制比其他作物更为复杂。利用正向遗传筛选技术，从水稻甲磺酸乙酯突变体库中筛选到一个对铝敏感的突变体，通过MutMap分析和互补试验，确定了铝敏感基因--铝相关蛋白激酶（ArPK），其表达受高浓度铝诱导。RNA测序结果显示arpk突变体精氨酸代谢途径功能紊乱，N-乙酰鸟氨酸脱乙酰酶（NAOD）表达下调，鸟氨酸脱羧酶1（ODC 1）表达上调。Al特异性地快速上调ODC 1的表达并导致腐胺（Put）的过度积累，而ODC抑制剂二氟甲基鸟氨酸（difluoromethylornithine）则逆转了arpk的Al敏感表型，表明内源Put的过度积累可能对根系生长有害，而ArPK似乎作为ODC 1作用的内源抑制剂，在Al处理下维持合适的内源Put水平。总之，我们确定了ArPK及其在控制一种新的ODC依赖的Put生物合成途径中的抑制作用，该途径特异性地影响水稻的铝抗性，从而丰富了对植物铝抗性的基本认识。
Rice, as one of the most aluminium (Al)‐resistant cereal crops, has developed more complicated Al resistance mechanisms than others. By using forward genetic screening from a rice ethyl methanesulfonate mutant library, we obtained a mutant showing specifically high sensitivity to Al. Through MutMap analysis followed by a complementation test, we identified the causal gene,Al‐related Protein Kinase(ArPK) for Al‐sensitivity.ArPKexpression was induced by a relatively longer exposure to high Al concentration in the roots. The result of RNA‐sequencing indicated the functional disorder in arginine metabolism pathway with downregulation ofN‐acetylornithine deacetylase(NAOD) expression and upregulation ofOrnithine decarboxylase1(ODC1) expression inarpkmutant. Al specifically and rapidly upregulatedODC1expression and causes overaccumulation of putrescine (Put), whereas the ODC inhibitor difluoromethylornithine reverted Al‐sensitive phenotype ofarpk, suggesting that overaccumulation of endogenous Put might be harmful for root growth, and thatArPKseems to act as an endogenous inhibitor of ODC1 action to maintain suitable endogenous Put level under Al treatment. Overall, we identifiedArPKand its putative repressive role in controlling a novel ODC‐dependent Put biosynthesis pathway specifically affecting rice Al resistance, thus enriching the fundamental understanding of plant Al resistance.