Histidine-Containing Dipeptides Mitigate Salt Stress in Plants by Scavenging Reactive Carbonyl Species
Histidine-Containing Dipeptides Mitigate Salt Stress in Plants by Scavenging Reactive Carbonyl Species
复制标题
含组氨酸二肽通过清除反应性羰基物质减轻植物中的盐胁迫
DOI：
10.1021/acs.jafc.2c03800
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发表时间：
2022
影响因子：
6.1
通讯作者：
Mano Jun’ichi
中科院分区：
文献类型：
--
作者：
Sultana Most. Sharmin;Yamamoto Shun-ichi;Biswas Md. Sanaullah;Sakurai Chisato;Isoai Hayato;Mano Jun’ichi
活性氧（ROS）是导致盐胁迫植物损伤的关键因素，但其在活细胞中的作用机制在很大程度上是未知的。我们研究了反应性羰基物种（RCS）的作用，即，过氧化脂质衍生的α，β-不饱和醛和酮在盐胁迫下植物生长迟缓中的作用。当拟南芥Col-0种子暴露于100 mM NaCl时，发芽延迟，幼苗中ROS、RCS和蛋白质羰基化水平增加。添加含有组氨酸的二肽肌肽、N-乙酰肌肽和鹅肌肽（据报道是RCS清除剂）恢复了发芽速度，抑制了RCS和蛋白质羰基化的增加，但不影响ROS水平。RCS丙烯醛、巴豆醛、（E）-2-戊烯醛和4-羟基-（E）-2-壬烯醛的水平的增加与发芽和生长抑制的延迟呈正相关。这些RCS，下游产生的ROS，因此主要负责植物的盐胁迫症状。
Reactive oxygen species (ROS) are critical factors that cause damage in salt-stressed plants, but their mechanisms of action in living cells are largely unknown. We investigated the roles of reactive carbonyl species (RCS), i.e., the lipid peroxide-derived α,β-unsaturated aldehydes and ketones, in plant growth retardation under salt stress. WhenArabidopsis thalianaCol-0 seeds were exposed to 100 mM NaCl, germination was delayed and the levels of ROS, RCS, and protein carbonylation in the seedlings were increased. Adding the histidine-containing dipeptides carnosine,N-acetylcarnosine, and anserine, which are reported RCS scavengers, restored the germination speed and suppressed the increases in RCS and protein carbonylation but did not affect the ROS level. Increases in the levels of the RCS acrolein, crotonaldehyde, (E)-2-pentenal, and 4-hydroxy-(E)-2-nonenal were positively correlated with the delay of germination and growth inhibition. These RCS, generated downstream of ROS, are thus primarily responsible for the salt-stress symptoms of plants.