Integrated Immunohistochemical Study on Small-Cell Carcinoma of the Lung Focusing on Transcription and Co-Transcription Factors.
Integrated Immunohistochemical Study on Small-Cell Carcinoma of the Lung Focusing on Transcription and Co-Transcription Factors.
复制标题
DOI：
10.3390/diagnostics10110949
复制
发表时间：
2020-11-13
期刊：
影响因子：
--
通讯作者：
Ito T
中科院分区：
文献类型：
--
作者：
Sato Y;Okamoto I;Kameyama H;Kudoh S;Saito H;Sanada M;Kudo N;Wakimoto J;Fujino K;Ikematsu Y;Tanaka K;Nishikawa A;Sakaguchi R;Ito T
小细胞肺癌（SCLC）是一种侵袭性恶性肿瘤，根据以下关键转录和共转录因子的表达分为四种亚型：ASCL1、NEUROD1、YAP1和POU2F3。这些关键分子的蛋白质表达水平可能对以分子亚型特异性方式形成SCLC特征很重要。我们期望这些分子的免疫组织化学（IHC）可以促进特定SCLC分子亚型的诊断，并有助于适当选择个性化治疗。我们尝试了从47个SCLC样本的基因表达综合数据集中选择的四个关键因子和26个候选SCLC靶分子的IHC，这些样本基于四个关键分子的阳性或阴性结果进行分组。我们检查了候选靶标和关键分子表达水平的差异。ASCL1在小细胞肺癌中的阳性率最高，ASCL1阳性组与阴性组之间某些靶分子的表达水平存在显著性差异。此外，这四个关键分子在SCLC细胞中协调和同时表达。对ASCL1阳性样本的IHC研究显示了许多候选SCLC靶分子，IHC可能成为确定SCLC分子亚型的必要方法。
Small-cell lung cancer (SCLC) is an aggressive malignant cancer that is classified into four subtypes based on the expression of the following key transcription and co-transcription factors: ASCL1, NEUROD1, YAP1, and POU2F3. The protein expression levels of these key molecules may be important for the formation of SCLC characteristics in a molecular subtype-specific manner. We expect that immunohistochemistry (IHC) of these molecules may facilitate the diagnosis of the specific SCLC molecular subtype and aid in the appropriate selection of individualized treatments. We attempted IHC of the four key factors and 26 candidate SCLC target molecules selected from the gene expression omnibus datasets of 47 SCLC samples, which were grouped based on positive or negative results for the four key molecules. We examined differences in the expression levels of the candidate targets and key molecules. ASCL1 showed the highest positive rate in SCLC samples, and significant differences were observed in the expression levels of some target molecules between the ASCL1-positive and ASCL1-negative groups. Furthermore, the four key molecules were coordinately and simultaneously expressed in SCLC cells. An IHC study of ASCL1-positive samples showed many candidate SCLC target molecules, and IHC could become an essential method for determining SCLC molecular subtypes.
登录
查看更多内容
影响因子：
8.8
作者：
Borromeo MD;Savage TK;Kollipara RK;He M;Augustyn A;Osborne JK;Girard L;Minna JD;Gazdar AF;Cobb MH;Johnson JE
通讯作者：
Johnson JE
影响因子：
5.2
作者：
Centonze G;Biganzoli D;Prinzi N;Pusceddu S;Mangogna A;Tamborini E;Perrone F;Busico A;Lagano V;Cattaneo L;Sozzi G;Roz L;Biganzoli E;Milione M
通讯作者：
Milione M
影响因子：
3.5
作者：
La Rosa, Stefano;Chiaravalli, Anna Maria;Capella, Carlo
通讯作者：
Capella, Carlo
影响因子：
6.4
作者：
Meder L;König K;Ozretić L;Schultheis AM;Ueckeroth F;Ade CP;Albus K;Boehm D;Rommerscheidt-Fuss U;Florin A;Buhl T;Hartmann W;Wolf J;Merkelbach-Bruse S;Eilers M;Perner S;Heukamp LC;Buettner R
通讯作者：
Buettner R
影响因子：
158.5
作者：
Horn, L.;Mansfield, A. S.;Liu, S. V.
通讯作者：
Liu, S. V.