The boundaries of the silenced HMR domain in Saccharomyces cerevisiae
The boundaries of the silenced HMR domain in Saccharomyces cerevisiae
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DOI：
10.1101/gad.13.6.698
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发表时间：
1999-03-15
影响因子：
10.5
通讯作者：
Kamakaka, RT
中科院分区：
文献类型：
--
作者：
Donze, D;Adams, CR;Kamakaka, RT
真核生物的染色体被组织成结构上和功能上离散的结构域，这些结构域提供了一种机制来压缩DNA以及描绘基因活性的独立单元。据信，绝缘子/边界元件分开这些结构域，在这里，我们报告的识别和表征边界元件侧翼转录抑制HMR基因座的酵母酿酒酵母。这些边界元素的删除导致沉默的染色质的扩散，而这些元素之间的沉默和启动子的异位插入阻断了沉默的HMR和端粒上的启动子的抑制作用。序列分析表明，边界元件包含TY 1 LTR，和tRNA基因和突变分析暗示Smc蛋白，编码染色体的结构成分，在边界元件的功能。
The chromosomes of eukaryotes are organized into structurally and functionally discrete domains that provide a mechanism to compact the DNA as well as delineate independent units of gene activity. It is believed that insulator/boundary elements separate these domains, Here we report the identification and characterization of boundary elements that flank the transcriptionally repressed HMR locus in the yeast Saccharomyces cerevisiae. Deletion of these boundary elements led to the spread of silenced chromatin, whereas the ectopic insertion of these elements between a silencer and a promoter blocked the repressive effects of the silencer on that promoter at HMR and at telomeres. Sequence analysis indicated that the boundary element contained a TY1 LTR, and a tRNA gene and mutational analysis has implicated the Smc proteins, which encode structural components of chromosomes, in boundary element function.