Overexpression of dimeric guanylyl cyclase cores of an atrial natriuretic peptide receptor.
Overexpression of dimeric guanylyl cyclase cores of an atrial natriuretic peptide receptor.
复制标题
心房钠尿肽受体二聚鸟苷酸环化酶核心的过度表达。
DOI：
10.1016/s0006-291x(05)81098-8
复制
发表时间：
1991
影响因子：
3.1
通讯作者：
Morkin,E
中科院分区：
文献类型：
--
作者：
Thorpe,DS;Niu,S;Morkin,E
利用细菌表达系统，产生并纯化了大量心房利钠肽受体胍基环化酶的催化核心。从含有尿素的缓冲液中重新折叠蛋白质后，酶具有正合作动力学，其Hill系数nH= 1.42±0.08。粒径隔离层析和变性聚丙烯酰胺凝胶电泳证实该酶是由具有相互作用催化位点的同型二聚体组成的。
Using a bacterial expression system, large amounts of the catalytic core of an atrial natriuretic peptide receptor guanylyl cyclase were produced and purified. After refolding the protein from a buffer containing urea, the enzyme had positively cooperative kinetics with a Hill coefficient, nH= 1.42 ± 0.08. Size exclusion chromatography and denaturing polyacrylamide gel electrophoresis demonstrated that the enzyme is composed of homodimers with interacting catalytic sites.