Population genetic analysis of Enterocytozoon bieneusi in humans
Population genetic analysis of Enterocytozoon bieneusi in humans
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DOI：
10.1016/j.ijpara.2012.01.003
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发表时间：
2012-03-01
影响因子：
4
通讯作者：
Xiao, Lihua
中科院分区：
文献类型：
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作者：
Li, Wei;Cama, Vitaliano;Xiao, Lihua
基于核糖体内转录间隔段序列分析的基因分型揭示了bieneusenterocyzoon的显著遗传多样性。到目前为止，还没有对布氏伊氏绦虫的群体遗传学及其在微孢子虫病流行病学中的意义进行研究。本研究利用秘鲁利马艾滋病患者的72份标本，在其内部转录间隔位点上分别分型为a、D、IV、EbpC、WL11、秘鲁7、秘鲁8、秘鲁10和秘鲁11，对其进行了多位点序列分型。72份标本共鉴定出39个多位点基因型。基因内连锁不平衡强烈，标记间遗传重组有限，表明该种群具有整体的无性系群体结构。对基因间连锁不平衡的测量和基于等位基因谱数据的标准化关联指数（I-A(S)）进一步支持了这一结论。基于序列和等位基因谱的系统发育分析显示，在研究群体中存在两个遗传分离群体，一个（第1组）包含人源性内转录间隔物基因型A的分离株，另一个（第2组）包含多种具有人源性内转录间隔物基因型（主要是基因型D和IV）的分离株。连锁失平衡和重组的测定表明，第2组为无性系群体结构，第1组为流行型群体结构。两个亚居群的形成经结构分析和Wright's固着指数（F-ST）分析证实。这些数据突出了MLST在理解比氏埃希菌流行病学方面的力量。由爱思唯尔有限公司代表澳大利亚寄生虫学学会出版。
Genotyping based on sequence analysis of the ribosomal internal transcribed spacer has revealed significant genetic diversity in Enterocytozoon bieneusi. Thus far, the population genetics of E. bieneusi and its significance in the epidemiology of microsporidiosis have not been examined. In this study, a multilocus sequence typing of E. bieneusi in AIDS patients in Lima, Peru was conducted, using 72 specimens previously genotyped as A, D, IV, EbpC, WL11, Peru7, Peru8, Peru10 and Peru11 at the internal transcribed spacer locus. Altogether, 39 multilocus genotypes were identified among the 72 specimens. The observation of strong intragenic linkage disequilibria and limited genetic recombination among markers were indicative of an overall clonal population structure of E. bieneusi. Measures of pair-wise intergenic linkage disequilibria and a standardised index of association (I-A(S)) based on allelic profile data further supported this conclusion. Both sequence-based and allelic profile-based phylogenetic analyses showed the presence of two genetically isolated groups in the study population, one (group 1) containing isolates of the anthroponotic internal transcribed spacer genotype A, and the other (group 2) containing isolates of multiple internal transcribed spacer genotypes (mainly genotypes D and IV) with zoonotic potential. The measurement of linkage disequilibria and recombination indicated group 2 had a clonal population structure, whereas group 1 had an epidemic population structure. The formation of the two sub-populations was confirmed by STRUCTURE and Wright's fixation index (F-ST) analyses. The data highlight the power of MLST in understanding the epidemiology of E. bieneusi. Published by Elsevier Ltd. on behalf of Australian Society for Parasitology Inc.