Evaluating the toxic effect of an antimicrobial agent on single bacterial cells with optical tweezers.
Evaluating the toxic effect of an antimicrobial agent on single bacterial cells with optical tweezers.
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DOI：
10.1364/boe.6.000112
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发表时间：
2015
影响因子：
3.4
通讯作者：
A. Samadi;Chensong Zhang;Joseph Chen;S. Reihani;Zhigang Chen
中科院分区：
文献类型：
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作者：
A. Samadi;Chensong Zhang;Joseph Chen;S. Reihani;Zhigang Chen
我们实施了一种光学镊子技术来评估化学制剂对单个细菌细胞的影响。作为原理的证明，捕获的大肠杆菌的生存能力是通过监测其鞭毛在不同浓度的乙醇存在下的运动性来确定的。我们发现，从捕捉器记录的位置时间序列的相关统计中可以有效地识别细菌的“杀死时间”，而直接从时间序列或相关功率谱中进行量化是困难的。我们的结果将细菌光损伤的致命影响降至最低，与以前使用传统培养方法的乙醇中毒报告一致。这种方法可用于研究药物和可移动细菌的其他配对组合，特别是以更高的精度测量单细胞的反应时间。
We implement an optical tweezers technique to assess the effects of chemical agents on single bacterial cells. As a proof of principle, the viability of a trapped Escherichia coli bacterium is determined by monitoring its flagellar motility in the presence of varying concentrations of ethyl alcohol. We show that the "killing time" of the bacterium can be effectively identified from the correlation statistics of the positional time series recorded from the trap, while direct quantification from the time series or associated power spectra is intractable. Our results, which minimize the lethal effects of bacterial photodamage, are consistent with previous reports of ethanol toxicity that used conventional culture-based methods. This approach can be adapted to study other pairwise combinations of drugs and motile bacteria, especially to measure the response times of single cells with better precision.