ABCtransporter mutations inCry1F-resistant fall armyworm (Spodoptera frugiperda) do not result in altered susceptibility to selected small molecule pesticides
ABCtransporter mutations inCry1F-resistant fall armyworm (Spodoptera frugiperda) do not result in altered susceptibility to selected small molecule pesticides
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DOI：
10.1002/ps.6106
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发表时间：
2020-10-10
影响因子：
4.1
通讯作者：
Jurat-Fuentes, Juan Luis
中科院分区：
文献类型：
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作者：
Abdelgaffar, Heba;Perera, Omaththage P.;Jurat-Fuentes, Juan Luis
背景从苏云金芽孢杆菌中获得Cry和Vip3杀虫蛋白的转基因作物可以有效地控制秋季粘虫。E·史密斯。然而，实习生的案例。西半球已有报告表明，转基因玉米产生了对Cry1F、Cry1Ab和Cry1A.105蛋白的抗性。重要的是，在波多黎各，果树对Cry1F玉米的抗性以前与对合成杀虫剂的敏感性较低有关。当鉴定时，对转基因玉米INS的抗性。果蝇涉及ABC转运蛋白亚家族C2(SfABCC2)基因的改变。这项工作的主要目的是测试导致对Cry1F产生抗性的SfABCC2突变在对合成和半合成小分子农药的敏感性中的作用。结果在Cry1F耐药株中检测到对联苯菊酯的敏感性边缘但显著降低，对司匹托仑的敏感性增加。来自波多黎各的果树携带SfABCC2基因移码突变。通过CRISPR/Cas9进行基因编辑，在实验室创建了一个SfABCC2基因敲除参考文献。果子树。与亲本对照相比，该基因敲除菌株对Cry1F的抗性提高了25倍，但对小分子农药的敏感性没有变化。结论SfABCC2基因突变引起的对Cry1F的抗性不会影响受试小分子农药的敏感性。
BACKGROUND Transgenic crops producing Cry and Vip3 insecticidal proteins from the bacteriumBacillus thuringiensisprovide effective control of the fall armyworm,Spodoptera frugiperdaJ. E. Smith. However, cases of practicalS. frugiperdaresistance to transgenic corn producing Cry1F, Cry1Ab and Cry1A.105 proteins have been reported in the Western hemisphere. Importantly,S. frugiperdaresistance to Cry1F corn in Puerto Rico was previously associated with lower susceptibility to synthetic pesticides. When characterized, resistance to transgenic corn inS. frugiperdainvolved alterations in an ABC transporter subfamily C2 (SfABCC2) gene. The main goal of this work was to test the role of mutations inSfABCC2that result in resistance to Cry1F in susceptibility to synthetic and semisynthetic small molecule pesticides. RESULTS Marginal but significantly reduced susceptibility to bifenthrin and increased susceptibility to spinetoram was detected in a Cry1F-resitantS. frugiperdastrain from Puerto Rico carrying a frameshift mutation in theSfABCC2gene. Gene editing by CRISPR/Cas9 created aSfABCC2knockout in a laboratory referenceS. frugiperdastrain. When compared to the parental reference, the knockout strain displayed 25-fold resistance to Cry1F but no alteration in susceptibility to small molecule pesticides. CONCLUSION These results support that resistance to Cry1F due to mutations in theSfABCC2gene do not affect susceptibility to the tested small molecule pesticides.