Two functionally distinct forms of the RSC nucleosome-remodeling complex, containing essential AT hook, BAH, and bromodomains
Two functionally distinct forms of the RSC nucleosome-remodeling complex, containing essential AT hook, BAH, and bromodomains
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DOI：
10.1016/s1097-2765(00)80382-2
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发表时间：
1999-11-01
期刊：
影响因子：
16
通讯作者：
Winston, F
中科院分区：
文献类型：
--
作者：
Cairns, BR;Schlichter, A;Winston, F
RSC 是来自酿酒酵母的一种必需的 15 种蛋白质核小体重塑复合物。我们已经确定了两个密切相关的 RSC 成员：Rsc1 和 Rsc2。生化分析显示 Rsc1 和 Rsc2 处于不同的复合物中，定义了 RSC 的两种形式。遗传分析表明Rsc1和Rsc2具有共同且独特的功能。 Rsc1 和 Rsc2 各自包含两个溴结构域、一个溴相邻同源 (BAH) 结构域和一个 AT 钩。溴结构域之一、BAH 结构域和 AT 钩对于 Rsc1 和 Rsc2 功能都是必需的，尽管它们不是组装成 RSC 复合物所必需的。因此，这些域是 RSC 功能所必需的。额外的遗传分析进一步证明 RSC 功能与转录控制相关。
RSC is an essential 15 protein nucleosome-remodeling complex from S. cerevisiae. We have identified two closely related RSC members, Rsc1 and Rsc2. Biochemical analysis revealed Rsc1 and Rsc2 in distinct complexes, defining two forms of RSC. Genetic analysis has shown that Rsc1 and Rsc2 possess shared and unique functions. Rsc1 and Rsc2 each contain two bromodomains, a bromo-adjacent homology (BAH) domain, and an AT hook. One of the bromodomains, the BAH domain, and the AT hook are each essential for Rsc1 and Rsc2 functions, although they are not required for assembly into RSC complexes. Therefore, these domains are required for RSC function. Additional genetic analysis provides further evidence that RSC function is related to transcriptional control.