Solid phase extraction technique-trends, opportunities and applications
Solid phase extraction technique-trends, opportunities and applications
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DOI：
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发表时间：
2006
影响因子：
1.8
通讯作者：
A. Żwir-Ferenc;M. Biziuk
中科院分区：
文献类型：
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作者：
A. Żwir-Ferenc;M. Biziuk
固相萃取（SPE）是一种使用固相和液相从溶液中分离一种或一种类型的分析物的萃取方法。它通常用于在使用色谱或其它分析方法定量样品中分析物的量之前净化样品。一般程序是将溶液加载到SPE相上，洗去不需要的组分，然后用另一种溶剂将所需的分析物冲洗到收集管中。固相萃取使用与液相色谱柱相同类型的固定相。固定相包含在玻璃或塑料柱中，位于玻璃料或玻璃棉上方。柱可以在固定相的顶部具有玻璃料，并且还可以具有旋塞阀以控制溶剂通过柱的流动。市售SPE柱的容量为1-10 ml，使用后丢弃。它通常用于在使用色谱或其它分析方法定量样品中分析物的量之前净化样品。固相萃取程序不仅用于从环境样品中提取痕量有机化合物，而且还用于去除复杂基质的干扰组分，以获得含有感兴趣的分析物的更清洁的提取物。SPE技术广泛应用于从液体基质和纯化提取物中分离分析物。本文综述了固相萃取技术的概况、最新发展趋势及其应用。
Solid phase extraction (SPE) is an extraction method that uses a solid phase and a liquid phase to isolate one, or one type, of analyte from a solution. it is usually used to clean up a sample before using a chromatographic or other analytical method to quantify the amount of analyte(s) in the sample. The general procedure is to load a solution onto the SPE phase, wash away undesired components, and then wash off the desired analytes with another solvent into a collection tube. Solid-phase extractions use the same type of stationary phases as are used in liquid chromatography columns. The stationary phase is contained in a glass or plastic column above a frit or glass wool. The column might have a frit on top of the stationary phase and might also have a stopcock to control the flow of solvent through the column. Commercial SPE cartridges have 1-10 ml capacities and are discarded after use. it is usually used to clean up a sample before using a chromatographic or other analytical method to quantify the amount of analyte(s) in the sample. Solid phase extraction procedures are used not only to extract traces of organic compounds from environmental samples but also to remove the interfering components of the complex matrices in order to obtain a cleaner extract containing the analytes of interest. The SPE technique is widely applied for isolation of analytes from a liquid matrix and purified extracts. This paper is a review of the literature regarding general information about SPE technique, new trends in SPE technique and its application.