Antioxidant responses and metal accumulation in tissues of Nile tilapia Oreochromis niloticus under Zn, Cd and Zn plus Cd exposures
Antioxidant responses and metal accumulation in tissues of Nile tilapia Oreochromis niloticus under Zn, Cd and Zn plus Cd exposures
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DOI：
10.1002/jat.1406
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发表时间：
2009-05-01
影响因子：
3.3
通讯作者：
Kargin, Ferit
中科院分区：
文献类型：
--
作者：
Firat, Oezguer;Cogun, Hikmet Y.;Kargin, Ferit
研究了锌、镉和锌+镉混合物对尼罗罗非鱼体内抗氧化指标和金属积累的影响。将鱼暴露于0.5和5.0 mg L(-1)锌、0.1和1.0 mg L镉、0.5 mg锌+0.1 mg L(-1)Cd和5.0 mg L(-1)锌+1.0 mg L(-1)Cd混合物中7和2 8d，测定鳃和肝脏中锌和镉的积累量、谷胱甘肽含量和葡萄糖-6-磷酸脱氢酶(G6PD)活性。随着暴露介质中金属浓度的增加和暴露时间的延长(较低浓度的锌除外)，金属在组织中的积累量增加。锌+镉组合暴露的鱼类组织中金属含量显著低于单一金属暴露的鱼类组织中的金属浓度。肝脏中金属积累量最高。重金属暴露影响了组织中的抗氧化指标，在锌、镉和锌+镉暴露下，鳃和肝脏中的GSH水平和G6PD活性都有所增加，尤其是在较高浓度下。这些抗氧化反应的增加在单独的Cd和与锌的组合中比单独使用锌的更高。此外，Cd单独暴露和Cd与Zn复合暴露时，GSH水平和G6PD活性均随暴露时间的延长而增加。结果表明，尼罗罗非鱼通过抗氧化机制抵抗重金属暴露所致的氧化应激。版权所有(C)2008 John Wiley&Sons，Ltd.
We investigated the effects of Zn, Cd and a Zn + Cd mixture on antioxidant parameters and metal accumulation in Oreochromis niloticus. Fish were exposed to 0.5 and 5.0 mg l(-1) Zn, 0.1 and 1.0 mg l(-1) Cd, and 0.5 mgl(-1) Zn + 0.1 mg l(-1) Cd and 5.0 mg l(-1) Zn + 1.0 mg l(-1) Cd mixtures for 7 and 28 days to determine Zn and Cd accumulation, reduced glutathione (GSH) level and glucose-6-phosphate dehydrogenase (G6PD) activity in gill and liver. There was increasing accumulation of the metals in the tissues with increasing concentrations of metals in the exposure medium and with increasing duration of exposure (except at the lower concentration of Zn). Concentration of metals in the tissues of fish exposed to the Zn + Cd combination were significantly lower than in fish exposed to the single metal. The highest metal accumulation was observed in the liver. Exposure to the heavy metals affected the antioxidant parameters in the tissues, with both GSH level and G6PD activity in the gill and liver being increased under Zn, Cd and Zn + Cd exposures, especially in their higher concentrations. These increases in the antioxidant responses were higher with the Cd alone, and in combination with Zn, than with Zn alone. Furthermore, GSH level and G6PD activity increased with increasing exposure period only for Cd alone, and in Cd combination with Zn. The results indicate that O. niloticus resisted oxidative stress induced by heavy metal exposure by antioxidant mechanisms. Copyright (C) 2008 John Wiley & Sons, Ltd.