Echocardiographic evaluation of the effects of stem cell therapy on perfusion and function in ischemic cardiomyopathy.
Echocardiographic evaluation of the effects of stem cell therapy on perfusion and function in ischemic cardiomyopathy.
复制标题
超声心动图评估干细胞疗法对缺血性心肌病的灌注和功能的影响。
DOI：
10.1016/j.echo.2013.10.011
复制
发表时间：
2014-02
影响因子：
6.5
通讯作者：
Lindner, Jonathan R.
中科院分区：
文献类型：
--
作者：
Inaba, Yoichi;Davidson, Brian P.;Kim, Sajeevani;Liu, Ya Ni;Packwood, William;Belcik, J. Todd;Xie, Aris;Lindner, Jonathan R.
缺血性左心室（LV）功能障碍的小动物模型对于干细胞治疗的临床前优化非常重要。我们假设，可以使用超声心动图成像评估小鼠细胞治疗后LV功能和区域灌注的时间变化。在永久性结扎左前降支动脉后7天和28天研究野生型小鼠（n=25）。在第7天，将动物随机接受闭胸超声引导的心肌内输注生理盐水（n=13）或5×105个多能成体祖细胞（MAPC）（n=12）。通过高频超声心动图测量左室舒张末期（LVEDV）和收缩末期（LVESV）容积、左室射血分数（LVEF）和每搏输出量。采用心肌声学造影（MCE）对灌注和缺损面积进行多平面评估。在第7天和第28天之间，MAPC处理的动物具有40-50%的缺损尺寸减小（p<0.001）和20-30%的总灌注增加（p<0.01）。未处理对照组的灌注未发生变化。两组中LVEDV和LVESV均在第7天和第28天之间增加，然而，与对照小鼠相比，MAPC处理的小鼠中LVESV增加程度较小（+4.2 7. 9 vs +19.2 22. 0 μL，p<0.05）。MAPC处理组小鼠的LVEF增加，对照组小鼠的LVEF降低（+3.0 ± 4.3 vs −5.6± 5.9%，p<0.01）。左室射血分数的变化与缺损面积大小或总灌注量的变化之间存在显着的线性关系。在缺血性LV功能障碍的小鼠模型中，高频超声心动图和MCE可用于评估对干细胞治疗的反应，并表征空间血流、心室功能和心室重构之间的关系。
Small animal models of ischemic left ventricular (LV) dysfunction are important for the pre-clinical optimization of stem cell therapy. We hypothesized that temporal changes in LV function and regional perfusion after cell therapy can be assessed in mice using echocardiographic imaging. Wild-type mice (n=25) were studied 7 and 28 days after permanent ligation of the left anterior descending artery. Animals were randomized to receive closed-chest ultrasound-guided intramyocardial delivery of saline (n=13) or 5×105 multipotential adult progenitor cells (MAPC) (n=12) at day 7. Left ventricular end-diastolic (LVEDV) and end-systolic (LVESV) volume, left ventricular ejection fraction (LVEF), and stroke volume were measured by high-frequency echocardiography. Multiplanar assessment of perfusion and defect area size were made by myocardial contrast echocardiography (MCE). Between day 7 and 28, MAPC-treated animals had a 40–50% reduction in defect size (p<0.001) and a 20–30% increase in total perfusion (p<0.01). Perfusion did not change in non-treated controls. Both LVEDV and LVESV increased between day 7 and 28 in both groups, however LVESV increased to a lesser degree in MAPC-treated versus control mice (+4.2±7.9 vs +19.2±22.0 μL, p<0.05). LVEF increased in the MAPC-treated mice and decreased in control mice (+3.0±4.3 vs −5.6±5.9 %, p<0.01). There was a significant linear relation between the change in LVEF and the change in either defect area size or total perfusion. High-frequency echocardiography and MCE in murine models of ischemic LV dysfunction can be used to assess the response to stem cell therapy and to characterize the relationship between spatial flow, ventricular function and ventricular remodeling.
登录
查看更多内容
影响因子：
24
作者：
Davidson, Brian P.;Kaufmann, Beat A.;Belcik, J. Todd;Xie, Aris;Qi, Yue;Lindner, Jonathan R.
通讯作者：
Lindner, Jonathan R.
DOI：
10.1161/atvbaha.109.198895
发表时间：
2010-06-01
影响因子：
8.7
作者：
Kumar, Arun H. S.;Caplice, Noel M.
通讯作者：
Caplice, Noel M.
DOI：
10.1016/j.jtcvs.2004.04.007
发表时间：
2004-08-01
影响因子：
6
作者：
Chachques, JC;Duarte, F;Carpentier, AF
通讯作者：
Carpentier, AF
影响因子：
24
作者：
Fujii, H;Tomita, S;Kitamura, S
通讯作者：
Kitamura, S
影响因子：
39.3
作者：
Grajek, Stefan;Popiel, Malgorzata;Komarnicki, Mieczyslaw
通讯作者：
Komarnicki, Mieczyslaw