The Distribution of TRPV1 and TRPV2 in the Rat Pharynx
The Distribution of TRPV1 and TRPV2 in the Rat Pharynx
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DOI：
10.1007/s10571-013-9938-3
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发表时间：
2013-07-01
影响因子：
4
通讯作者：
Ichikawa, Hiroyuki
中科院分区：
文献类型：
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作者：
Sasaki, Rika;Sato, Tadasu;Ichikawa, Hiroyuki
免疫组织化学的两个伤害性传感器，瞬时受体电位阳离子通道V亚家族成员1（TRPV 1）和2（TRPV 2），进行咽及其邻近地区。TRPV 1-免疫反应（IR）检测到神经纤维下方和内的上皮和/或味蕾样结构。在咽部，这些神经纤维丰富的鼻口部和鼻口部和喉部的边界区域。在会厌的喉侧和软腭也有很多。TRPV 2-IR在咽部和会厌中以及舌根和软腭中由树突状细胞表达。这些细胞位于上皮和固有层。TRPV 2免疫反应（IR）树突状细胞在鼻咽部，会厌和舌头。TRPV 2-IR树突状突起的缺失通常掩盖了这些部分中TRPV 2-IR神经纤维的存在。但在软腭上皮中可见TRPV 2阳性神经纤维。逆行追踪法还发现，支配咽或软腭的感觉神经元在颈-岩神经节复合体中丰富，而在结状神经节中相对较少。在颈-岩神经节复合体中，三分之一的咽和软腭神经元表达TRPV 1-IR和TRPV 2-IR。TRPV 2-IR在复合体中的咽神经元和软腭神经元中的阳性率分别为11.5%和30.9%。TRPV 1和CGRP在咽部和软腭神经元中的共表达较常见。本研究表明，TRPV 1-和TRPV 2-IR颈岩神经元可能与吞咽反射的调节。
Immunohistochemistry for two nociceptive transducers, the transient receptor potential cation channel subfamily V members 1 (TRPV1) and 2 (TRPV2), was performed on the pharynx and its adjacent regions. TRPV1-immunoreactivity (IR) was detected in nerve fibers beneath and within the epithelium and/or taste bud-like structure. In the pharynx, these nerve fibers were abundant in the naso-oral part and at the border region of naso-oral and laryngeal parts. They were also numerous on the laryngeal side of the epiglottis and in the soft palate. TRPV2-IR was expressed by dendritic cells in the pharynx and epiglottis, as well as in the root of the tongue and soft palate. These cells were located in the epithelium and lamina propria. TRPV2-immunoreactive (IR) dendritic cells were numerous in the naso-oral part of the pharynx, epiglottis, and tongue. Abundance of TRPV2-IR dendritic processes usually obscured the presence of TRPV2-IR nerve fibers in these portions. However, some TRPV2-IR nerve fibers could be observed in the epithelium of the soft palate. Retrograde tracing method also revealed that sensory neurons which innervate the pharynx or soft palate were abundant in the jugular-petrosal ganglion complex and relatively rare in the nodose ganglion. In the jugular-petrosal ganglion complex, TRPV1- and TRPV2-IR were expressed by one-third of pharyngeal and soft palate neurons. TRPV2-IR was also detected in 11.5 % pharyngeal and 30.9 % soft palate neurons in the complex. Coexpression of TRPV1 and CGRP was frequent among pharyngeal and soft palate neurons. The present study suggests that TRPV1- and TRPV2-IR jugular-petrosal neurons may be associated with the regulation of the swallowing reflex.