Elongation of Ganglioside (GD1a) Micellar Structure Depending on Cholesterol Content
Elongation of Ganglioside (GD1a) Micellar Structure Depending on Cholesterol Content
复制标题
神经节苷脂 (GD1a) 胶束结构的伸长取决于胆固醇含量
DOI：
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发表时间：
2001
期刊：
影响因子：
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通讯作者：
M. Hirai
中科院分区：
文献类型：
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作者：
T. Hayakawa;M. Hirai
摘要利用同步辐射小角X射线散射（SR-SAXS）研究了不同摩尔分数胆固醇对二唾液酸神经节苷脂（GD 1a）/胆固醇混合胶束结构的影响。的胶束的回转半径（Rg），估计从所获得的数据，增加胆固醇含量。Hirai等人（1996）成功应用于GD 1胶束结构估计的胶束多壳模型分析表明，胶束的Rg的胆固醇依赖性增加是由于其长球形胞体结构的延长。在目前的分析中，我们假设胶束的神经节苷脂/胆固醇混合物作为一个三层壳结构，考虑到高电子密度的类固醇环的胆固醇，插入在胶束的疏水区域。所检查的[神经节苷脂]/[胆固醇]的摩尔比为1：0、1：0.05、1：0.1、1：0.2、1：0.4、1：0.7和1：1。所有样品的神经节苷脂浓度恒定在0.5%w/v。
Abstract The influence of cholesterol with various molar fractions on the structure of disialoganglioside (GD1a)/cholesterol mixed micelle was investigated by using synchrotron radiation small-angle X-ray scattering (SR-SAXS). The radius of gyration (Rg) of the micelles, estimated from the obtained data, was increased with increasing cholesterol content. A multi-shell model analysis for micelles, that was successfully applied to the structure estimation of GD1 micelles by Hirai et al. (1996), revealed that the cholesterol-dependent increase of Rg of the micelles is due to an elongation of its prolate spherocylindrical structure. In the present analysis, we assumed the micelle of the ganglioside/cholesterol mixture as a triple-shell structure taking into account the high electron density of the steroid ring of cholesterol that is inserted in the hydrophobic region of the micelle. The examined molar ratios of [ganglioside]/[cholesterol] were 1:0, 1:0.05, 1:0.1, 1:0.2, 1:0.4, 1:0.7 and 1:1. The ganglioside concentration for all samples was constant at 0.5 % w/v.
影响因子：
2.9
作者：
Maulik, PR;Shipley, GG
通讯作者：
Shipley, GG