Viable SARS-CoV-2 in the air of a hospital room with COVID-19 patients
Viable SARS-CoV-2 in the air of a hospital room with COVID-19 patients
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DOI：
10.1016/j.ijid.2020.09.025
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发表时间：
2020-11-01
影响因子：
8.4
通讯作者：
Wu, Chang-Yu
中科院分区：
文献类型：
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作者：
Lednickya, John A.;Lauzardo, Michael;Wu, Chang-Yu
目的：由于在气溶胶中检测到SARS-CoV-2 RNA但未能分离出活的（传染性）病毒的报道很常见，因此严重急性呼吸道综合征冠状病毒2（SARS-CoV-2）是否可以通过气溶胶传播存在很大争议。这个难题的发生是因为普通的空气采样器可以通过其苛刻的收集过程来捕获病毒粒子。我们试图解决这个问题是否可行的SARS-CoV-2可以出现在气溶胶中使用VIVAS空气采样器，操作在温和的水蒸气冷凝principle.Methods的问题：空气样本收集在两个2019冠状病毒病（COVID-19）患者的病房，一个准备出院，另一个新入院，进行RT-qPCR和病毒培养。结果：从距患者2 ~ 4.8m的空气中分离到SARS-CoV-2活病毒，经测序证实为SARS-CoV-2。从空气采样器收集的材料中分离出的SARS-CoV-2菌株的基因组序列与从新入院患者中分离出的序列相同。活病毒浓度的估计值范围为6至74 TCID 50单位/L的空气。结论：在没有气溶胶生成程序的情况下，患有COVID-19呼吸道症状的患者会产生含有活性SARS-CoV-2的气溶胶，这些气溶胶可能是病毒传播的来源。O 2020作者。由Elsevier Ltd代表国际传染病学会出版。这是一个在CC BY-NC-ND许可证下的开放获取文章（http：//creativecommons.org/licenses/by-nc-nd/4.0/）。
Objectives: Because the detection of SARS-CoV-2 RNA in aerosols but failure to isolate viable (infectious) virus are commonly reported, there is substantial controversy whether severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can be transmitted through aerosols. This conundrum occurs because common air samplers can inactivate virions through their harsh collection processes. We sought to resolve the question whether viable SARS-CoV-2 can occur in aerosols using VIVAS air samplers that operate on a gentle water vapor condensation principle.Methods: Air samples collected in the hospital room of two coronavirus disease-2019 (COVID-19) patients, one ready for discharge and the other newly admitted, were subjected to RT-qPCR and virus culture. The genomes of the SARS-CoV-2 collected from the air and isolated in cell culture were sequenced.Results: Viable SARS-CoV-2 was isolated from air samples collected 2 to 4.8 m away from the patients. The genome sequence of the SARS-CoV-2 strain isolated from the material collected by the air samplers was identical to that isolated from the newly admitted patient. Estimates of viable viral concentrations ranged from 6 to 74 TCID50 units/L of air.Conclusions: Patients with respiratory manifestations of COVID-19 produce aerosols in the absence of aerosol-generating procedures that contain viable SARS-CoV-2, and these aerosols may serve as a source of transmission of the virus.O 2020 The Author(s). Published by Elsevier Ltd on behalf of International Society for Infectious Diseases. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).