Identification of genotypes of Giardia intestinalis of human isolates in Egypt
Identification of genotypes of Giardia intestinalis of human isolates in Egypt
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DOI：
10.1007/s00436-008-1113-2
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发表时间：
2008-10-01
影响因子：
2
通讯作者：
Mas-Coma, S.
中科院分区：
文献类型：
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作者：
Foronda, P.;Bargues, M. D.;Mas-Coma, S.
在埃及，人类肠贾第鞭毛虫的基因分型研究有限。为了确定肠胃炎的流行情况，从埃及采集了粪便样本。样品用密度梯度离心法浓缩。对标本进行TPI基因的聚合酶链式反应和DNA序列分析。在52名受检者中，贾第虫感染率为34.6%。DNA序列分析表明，B组合是最常见的(80%)基因，15%的阳性样本属于E组合，5%的阳性样本属于A组合。当然，A和B两种基因在世界范围内都是非常常见的。然而，到目前为止，还没有人知道E集合体对人类具有传染性。因此，这是首次在人类中发现E蛋白。然而，需要对第二个基因座进行进一步分析才能证实这一结果。埃及感染贾第虫的牛可能在多大程度上构成人类感染的风险尚不清楚。
In Egypt, the genotyping study of Giardia intestinalis in human is limited. To determine the prevalence of G. intestinalis, faecal samples were collected from Egypt. Samples were concentrated using density gradient centrifugation. The samples were subjected to PCR and DNA sequence analysis for TPI gene. Prevalence of Giardia infection was 34.6% of 52 examined. DNA sequence showed that the Assemblage B was the most prevalent (80%) genotype, the 15% of the positive samples belonged to Assemblage E, and the 5% of them belonged to Assemblage A. Certainly, both genotypes A and B are highly common in human worldwide. However, up to now, Assemblage E had not been known to be infectious for humans. Therefore, this is the first time that Assemblage E is reported in human. However, further analyses of a second locus are required to confirm this result. The extent to which Giardia-infected cattle in Egypt might pose a risk of human infection is unknown.