Effects of Vitamin A (Retinol) Release from Calcium Phosphate Matrices and Porous 3D Printed Scaffolds on Bone Cell Proliferation and Maturation.
Effects of Vitamin A (Retinol) Release from Calcium Phosphate Matrices and Porous 3D Printed Scaffolds on Bone Cell Proliferation and Maturation.
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DOI：
10.1021/acsabm.1c01181
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发表时间：
2022-03-21
影响因子：
4.7
通讯作者：
Bose, Susmita
中科院分区：
文献类型：
--
作者：
Vu, Ashley A;Kushram, Priya;Bose, Susmita
维生素A是一种脂溶化合物，对视力健康广为人知。关于其对骨健康的影响的报道差异很大，需要进一步研究才能真正了解其对骨细胞增殖的作用。视黄醇是维生素A的一种生物活性形式，被引入合成骨移植支架中，用于低负荷的临床骨治疗。这项工作的目的是了解视黄醇在钙磷酸盐基质中对成骨细胞和破骨细胞的影响，包括相互连接的多孔3D打印磷酸三钙支架。结果表明，当聚己内酯和聚乙二醇作为药物载体时，疏水性视黄醇可以从骨支架中释放出来。视黄醇的体外释放可以支持成骨细胞(骨形成)细胞增殖增加20%±1%，并有适当的细胞黏附和丝状突起。当视黄醇存在时，破骨细胞形态发生坏死和撕裂，增殖减少约6±1%。此外，扫描电子显微镜观察到破骨细胞吸收凹槽的抑制作用。由于支架的圆孔互连有助于视黄醇的释放，该系统可以提供传统骨移植的替代方案，同时还可以通过促进成骨细胞增殖和抑制破骨细胞的吸收活动来支持骨愈合。
Vitamin A is a fat-soluble compound widely known for vision health. Highly variable reports on its effects on bone health have necessitated further research to truly understand its role on bone cell proliferation. Retinol, one bioactive form of vitamin A, is incorporated into synthetic bone graft scaffolds for low load-bearing clinical bone treatment. The objective of this work is to understand the effects of retinol on osteoblast and osteoclast cells when embedded within calcium phosphate matrices, including interconnected porous 3D printed tricalcium phosphate scaffolds. Results show that hydrophobic retinol can be released from bone scaffolds when a combination of biodegradable polymers, polycaprolactone and polyethylene glycol, are employed as drug carriers. The release of retinol in vitro can support a 20 ± 1% increase in osteoblast (bone-forming) cell proliferation with proper cell adhesion and filopodial extensions. Osteoclast cell morphology is necrosed and torn with a reduction in proliferation at approximately 6 ± 1% when retinol is present. In addition, inhibition of osteoclastic resorption pit bays is noted using scanning electron microscopy. With the scaffolds’ round pore interconnectivity facilitating retinol release, this system can provide an alternative to traditional bone grafts while additionally supporting bone healing through enhanced osteoblast cell proliferation and inhibition of osteoclast resorption activity.