Searching for plasticity in dissociated cortical cultures on multi-electrode arrays.
Searching for plasticity in dissociated cortical cultures on multi-electrode arrays.
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DOI：
10.1186/1477-5751-5-16
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发表时间：
2006-10-26
期刊：
影响因子：
--
通讯作者：
Potter SM
中科院分区：
文献类型：
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作者：
Wagenaar DA;Pine J;Potter SM
我们试图通过嵌入培养皿底物的细胞外电极(多电极阵列，或MEA)的刺激，在密集的皮质细胞培养中诱导功能可塑性。我们遵循了几种与文献中使用的相关的诱导方案以及一些新的诱导方案，寻找在自发爆发模式的变化和阵列对电刺激的反应模式中表达的可塑性。实验是通过分布式电刺激或升高细胞外镁浓度来抑制整个培养范围的自发爆发，以及在未经处理的情况下进行。伴随诱导而来的变化在幅度上并不比自发发生的变化大，但在一种新的方案中，使用分布式电刺激来平息自发爆发。
We attempted to induce functional plasticity in dense cultures of cortical cells using stimulation through extracellular electrodes embedded in the culture dish substrate (multi-electrode arrays, or MEAs). We looked for plasticity expressed in changes in spontaneous burst patterns, and in array-wide response patterns to electrical stimuli, following several induction protocols related to those used in the literature, as well as some novel ones. Experiments were performed with spontaneous culture-wide bursting suppressed by either distributed electrical stimulation or by elevated extracellular magnesium concentrations as well as with spontaneous bursting untreated. Changes concomitant with induction were no larger in magnitude than changes that occurred spontaneously, except in one novel protocol in which spontaneous bursts were quieted using distributed electrical stimulation.